COMPARISON OF CULTURE AND MULTIPLEX PCR IN DETECTION OF FASTIDIOUS BACTERIA ASSOCIATED WITH OTITIS MEDIA AMONG SUSPECTED PATIENT ADMITTED TO AMIR-ALAM HOSPITAL

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DELSOUZ BAHRI TINA; GOUDARZI MEHDI; DABIRI SATRI SASAN; EBRAHIMI NASRIN; ASADI AREZOO; GHAFOORI SEYED MOHAMMAD; HOURI HAMIDREZA; GOUDARZI HOSSEIN

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Journal: Research in Medicine Year:2017 | Volume:40 | Issue:4 Page(s): 192-196

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Title

COMPARISON OF CULTURE AND MULTIPLEX PCR IN DETECTION OF FASTIDIOUS BACTERIA ASSOCIATED WITH OTITIS MEDIA AMONG SUSPECTED PATIENT ADMITTED TO AMIR-ALAM HOSPITAL

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Keywords

OTITIS MEDIAQ2

ALLOIOCOCCUS OTITIDISQ2

HAEMOPHILUS INFLUENZAEQ2

MORAXELLA CATARRHALISQ2

MULTIPLEX PCRQ2

Abstract

Background: OTITIS MEDIA (OM) is a common disease among children. Various factors, including bacteria and viruses are the cause of OM. The most common bacterial pathogens that can cause OM are Streptococcus pneumoniae, HAEMOPHILUS INFLUENZAE, MORAXELLA CATARRHALIS and Streptococcus pyogenes. Recently, ALLOIOCOCCUS OTITIDIS is known as one of the causes of OM. For some reasons such as fastidious growth and difficulty in sampling, few studies have been completed on this bacterium. The aim of this study was comparison of culture and MULTIPLEX PCR in detection of fastidious bacterial pathogens associated with OTITIS MEDIA.Material & Methods: In this descriptive studies, during a period of 5 month from March 2013 to July 2014, 50 middle ear discharge specimens were collected from patients suspected with OTITIS MEDIA in Amir-Alam Hospital. Samples were assessed by culture and MULTIPLEX PCR method.Results: In the study, 3 strains of ALLOIOCOCCUS OTITIDIS (6%), 3 strains of HAEMOPHILUS INFLUENZAE (6%) and 1 strain of MORAXELLA CATARRHALIS (2%) were isolated by culture. Also, by MULTIPLEX PCR method 25 ALLOIOCOCCUS OTITIDIS (50%), 28 HAEMOPHILUS INFLUENZAE (56%) and 11 MORAXELLA CATARRHALIS (22%) were detected in the samples.Conclusion: Identifying fastidious bacteria with culture method is difficult, but MULTIPLEX PCR method is more accurate, faster and more sensitive of culture method and biochemical tests and can be done quickly.

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APA: Copy

DELSOUZ BAHRI, TINA, GOUDARZI, MEHDI, DABIRI SATRI, SASAN, EBRAHIMI, NASRIN, ASADI, AREZOO, GHAFOORI, SEYED MOHAMMAD, HOURI, HAMIDREZA, & GOUDARZI, HOSSEIN. (2017). COMPARISON OF CULTURE AND MULTIPLEX PCR IN DETECTION OF FASTIDIOUS BACTERIA ASSOCIATED WITH OTITIS MEDIA AMONG SUSPECTED PATIENT ADMITTED TO AMIR-ALAM HOSPITAL. PAJOUHESH DAR PEZESHKI, 40(4), 192-196. SID. https://sid.ir/paper/41568/en

Vancouver: Copy

DELSOUZ BAHRI TINA, GOUDARZI MEHDI, DABIRI SATRI SASAN, EBRAHIMI NASRIN, ASADI AREZOO, GHAFOORI SEYED MOHAMMAD, HOURI HAMIDREZA, GOUDARZI HOSSEIN. COMPARISON OF CULTURE AND MULTIPLEX PCR IN DETECTION OF FASTIDIOUS BACTERIA ASSOCIATED WITH OTITIS MEDIA AMONG SUSPECTED PATIENT ADMITTED TO AMIR-ALAM HOSPITAL. PAJOUHESH DAR PEZESHKI[Internet]. 2017;40(4):192-196. Available from: https://sid.ir/paper/41568/en

IEEE: Copy

TINA DELSOUZ BAHRI, MEHDI GOUDARZI, SASAN DABIRI SATRI, NASRIN EBRAHIMI, AREZOO ASADI, SEYED MOHAMMAD GHAFOORI, HAMIDREZA HOURI, and HOSSEIN GOUDARZI, “COMPARISON OF CULTURE AND MULTIPLEX PCR IN DETECTION OF FASTIDIOUS BACTERIA ASSOCIATED WITH OTITIS MEDIA AMONG SUSPECTED PATIENT ADMITTED TO AMIR-ALAM HOSPITAL,” PAJOUHESH DAR PEZESHKI, vol. 40, no. 4, pp. 192–196, 2017, [Online]. Available: https://sid.ir/paper/41568/en

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