مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

MACROPHAGE IMMUNE RESPONSE SUPPRESSION BY RECOMBINANTMYCOBACTERIUM TUBERCULOSIS ANTIGENS, THE ESAT-6, CFP-10, AND ESAT-6/CFP-10 FUSION PROTEINS

Pages

  296-304

Abstract

 Background: Macrophage immune responses are affected by the secretory proteins of MYCOBACTERIUM TUBERCULOSIS (Mtb). This study aimed to examine the immune responses of macrophages to Mtb secretory antigens, namely ESAT-6, CFP-10, and ESAT-6/ CFP-10.Methods: THP-1 cells (a human monocytic cell line) were cultured and differentiated to macrophages by phorbol 12-myristate 13-acetate. The cytotoxicity of the recombinant Mtbproteins was assessed using the MTT assay. Two important immune responses of macrophages, namely NO and ROS production, were measured in response to the ESAT-6, CFP-10, and ESAT-6/CFP-10 antigens. The data were analyzed using one-way ANOVA with SPSS, version 16, and considered significant at P<0.05.Results: The results showed that the ESAT-6, CFP-10, and ESAT-6/CFP-10 proteins markedly reduced macrophage immune response. The treatment of the THP-1-differentiated cells with ESAT-6, CFP-10, and ESAT-6/CFP-10 reduced NO and ROS production. The treated THP-1-differentiated cells exhibited less inducible NO synthase activity than did the untreated cells. No toxic effect on macrophage viability was observed for the applied proteins at the different concentrations.Conclusion: It seems that the decline in macrophage immune response is due to the suppression of NO and ROS production pathways without any effect on cell viability.

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    APA: Copy

    SEGHATOLESLAM, ATEFEH, HEMMATI, MINA, EBADAT, SAEEDEH, MOVAHEDI, BAHRAM, & MOSTAFAVI POUR, ZOHREH. (2016). MACROPHAGE IMMUNE RESPONSE SUPPRESSION BY RECOMBINANTMYCOBACTERIUM TUBERCULOSIS ANTIGENS, THE ESAT-6, CFP-10, AND ESAT-6/CFP-10 FUSION PROTEINS. IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS), 41(4), 296-304. SID. https://sid.ir/paper/276546/en

    Vancouver: Copy

    SEGHATOLESLAM ATEFEH, HEMMATI MINA, EBADAT SAEEDEH, MOVAHEDI BAHRAM, MOSTAFAVI POUR ZOHREH. MACROPHAGE IMMUNE RESPONSE SUPPRESSION BY RECOMBINANTMYCOBACTERIUM TUBERCULOSIS ANTIGENS, THE ESAT-6, CFP-10, AND ESAT-6/CFP-10 FUSION PROTEINS. IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS)[Internet]. 2016;41(4):296-304. Available from: https://sid.ir/paper/276546/en

    IEEE: Copy

    ATEFEH SEGHATOLESLAM, MINA HEMMATI, SAEEDEH EBADAT, BAHRAM MOVAHEDI, and ZOHREH MOSTAFAVI POUR, “MACROPHAGE IMMUNE RESPONSE SUPPRESSION BY RECOMBINANTMYCOBACTERIUM TUBERCULOSIS ANTIGENS, THE ESAT-6, CFP-10, AND ESAT-6/CFP-10 FUSION PROTEINS,” IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS), vol. 41, no. 4, pp. 296–304, 2016, [Online]. Available: https://sid.ir/paper/276546/en

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