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Information Journal Paper

Title

INTRODUCTION OF THREE INDEPENDENT SELECTION MARKERS IN LEISHMANIA

Pages

  13-18

Keywords

HSV-THYMIDINE KINASE (HSV-TK) 
CYTOSINE DEAMINASE (CD) 
GANCICLOVIR (GCV) 
5-FLUOROCYTOSINE (5-FC) 
NOURSEOTHERICIN (NS) 

Abstract

 The pLE2SCX vector was developed for the stable expression of exogenous genes in the protozoan parasite Leishmania. The pLE2SCX construct contains three independent selection markers: herpes simplex virus thymidine kinase (HSV-TK), cytosine deaminase (CD) and streptothericin acetyltransferase gene (sat) in multiple cloning site, flanking by 5’ and 3’ untranslated regions of the previously cloned Leishmania major hexa-binding protein gene. Selection was based on resistance to the nourseothericin (Ns) which corresponds to sat gene. The two negative selection; HSV-TK and CD genes, make the transformed cell sensitive to ganciclovir (GCV) and 5-fluorocytosine (5-FC). The vector was introduced into Leishmania promastigotes by electroporation and maintained as circular form. The selected transfectants were not grown on media with GCV or 5-FC. Using two drug sensitive selectable markers together on a vector is a novel strategy in gene cloning in Leishmania. This stable transfection vector has allowed the permanently expression of several different exogenous genes at the same time in Leishmania

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    APA: Copy

    DAVOUDI, N., MAHBOUDI, F., AZIZI, MOHAMMAD, ADELI, A., & MCMASTER, R.W.. (2003). INTRODUCTION OF THREE INDEPENDENT SELECTION MARKERS IN LEISHMANIA. IRANIAN BIOMEDICAL JOURNAL, 7(1), 13-18. SID. https://sid.ir/paper/276984/en

    Vancouver: Copy

    DAVOUDI N., MAHBOUDI F., AZIZI MOHAMMAD, ADELI A., MCMASTER R.W.. INTRODUCTION OF THREE INDEPENDENT SELECTION MARKERS IN LEISHMANIA. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2003;7(1):13-18. Available from: https://sid.ir/paper/276984/en

    IEEE: Copy

    N. DAVOUDI, F. MAHBOUDI, MOHAMMAD AZIZI, A. ADELI, and R.W. MCMASTER, “INTRODUCTION OF THREE INDEPENDENT SELECTION MARKERS IN LEISHMANIA,” IRANIAN BIOMEDICAL JOURNAL, vol. 7, no. 1, pp. 13–18, 2003, [Online]. Available: https://sid.ir/paper/276984/en

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