CYTOTOXICITY EFFECT OF CLADRIBINE ON THE MCF-7 HUMAN BREAST CANCER CELL LINE

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HASHEMI M.; KARAMI TEHRANI FATEMEH; GHAVAMI S.

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Journal: Iranian Biomedical Journal Year:2004 | Volume:8 | Issue:1 Page(s): 7-12

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Title

CYTOTOXICITY EFFECT OF CLADRIBINE ON THE MCF-7 HUMAN BREAST CANCER CELL LINE

Author(s)

HASHEMI M. | KARAMI TEHRANI FATEMEH | GHAVAMI S. | Issue Writer Certificate

Keywords

CLADRIBINE

APOPTOSIS

MCF-7

CASPASE-3

CASPASE-9

Abstract

CLADRIBINE, an analogue of deoxyadenosine, is highly toxic for both non-dividing and proliferating cells and has shown activity in the treatment of several malignancies. Therefore, the aim of the present study is to investigate the cytotoxicity effect of CLADRIBINE (2-CdA) on the breast cancer cell line, MCF-7 (estrogen receptor positive, ER+). MTT assay, annexin V-Fluorescein/PI and Hoechst 33258 staining were used to detect cytotoxicity and cell APOPTOSIS. The activation of CASPASE-3 and -9 was assayed using caspase activation assay kits. Gel electrophoresis was performed to detect DNA fragmentation. Treatment of MCF-7 cells with different concentrations of 2-CdA resulted in a significant increase in the cell death. Annexin V-Fluorescein/PI and Hoechst 33258 staining revealed that the cell death was mainly an apoptotic type. A significant (p<0.05) increase in the activity of CASPASE-9 was observed but CASPASE-3 activity was unchanged and DNA laddering profile was not obtained. Pre-treatment of the cells with kinase inhibitor, 5¢-amino-5¢-deoxyadenosine inhibited the cytotoxicity effect of CLADRIBINE. In conclusion, this study has shown that high dose of CLADRIBINE (higher than 25 mM) has an apoptotic effect on MCF-7 cells and that its intracellular phosphorylation is necessary.

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APA: Copy

HASHEMI, M., KARAMI TEHRANI, FATEMEH, & GHAVAMI, S.. (2004). CYTOTOXICITY EFFECT OF CLADRIBINE ON THE MCF-7 HUMAN BREAST CANCER CELL LINE . IRANIAN BIOMEDICAL JOURNAL, 8(1), 7-12. SID. https://sid.ir/paper/277000/en

Vancouver: Copy

HASHEMI M., KARAMI TEHRANI FATEMEH, GHAVAMI S.. CYTOTOXICITY EFFECT OF CLADRIBINE ON THE MCF-7 HUMAN BREAST CANCER CELL LINE . IRANIAN BIOMEDICAL JOURNAL[Internet]. 2004;8(1):7-12. Available from: https://sid.ir/paper/277000/en

IEEE: Copy

M. HASHEMI, FATEMEH KARAMI TEHRANI, and S. GHAVAMI, “CYTOTOXICITY EFFECT OF CLADRIBINE ON THE MCF-7 HUMAN BREAST CANCER CELL LINE ,” IRANIAN BIOMEDICAL JOURNAL, vol. 8, no. 1, pp. 7–12, 2004, [Online]. Available: https://sid.ir/paper/277000/en

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