IN VITRO INTERACTION AND COLOCALIZATION OF HSV-1 ORF P WITH A CELLULAR SPLICING FACTOR (SC35) USING PULLDOWN ASSAY

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

KARIMI A.; SALEHI R.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Biomedical Journal Year:2005 | Volume:9 | Issue:2 Page(s): 67-71

Download Full-Text

View:

321

Download:

161

Cites:

Information Journal Paper

Title

IN VITRO INTERACTION AND COLOCALIZATION OF HSV-1 ORF P WITH A CELLULAR SPLICING FACTOR (SC35) USING PULLDOWN ASSAY

Author(s)

KARIMI A. | SALEHI R. | Issue Writer Certificate

Keywords

OPEN READING FRAME (ORF P)

INFECTED CELL PROTEIN (ICP) 34.5

LATENCY ASSOCIATED TRANSCRIPT (LAT)

SPLICING COMPONENT (SC) 35

Abstract

Herpes simplex virus type-1 (HSV-1) causes a variety of diseases in human. This virus is a neurotropic pathogen of human that establishes latent infection in the sensory ganglia innervating the site of primary infection. A number of genes including ICP34.5 control HSV-1 pathogenicity and ICP34.5 has been identified as HSV-1 virulence gene. Open reading frame P (ORF P) is also a HSV-1 gene that might have a role in latency. A complication in the analysis of the role of ICP34.5 and ORF P in the HSV-1 life cycle is that these two are overlapping antisense genes. ORF P is also deleted in ICP34.5 negative mutants and to date, no definite function is attributed to it. To attribute characteristics which were originally attributed solely to ICP34.5 to each of these two genes (ORF P or ICP34.5), an approach is to construct a number of HSV-1 recombinant viruses that express ICP34.5 and ORF P independently. An alternative way is to determine if ORF P interacts with any of the cellular and viral proteins both in vitro and in vivo. Using Glutathione-S-transferase (GST) pulldown assay and Western-blotting, we showed that ORF P interacts with a cellular splicing factor (SC35) in vitro. To investigate the colocalization of ORF P and SC35, nuclear and cytoplasmic fractionation of ORF P/SC35 was also carried out. Our results showed that both SC35 and ORF P are located in the nucleus of HSV-1 infected cells. Conclusively, because ORF P interacts and colocalizes with SC35, it might have a role in splicing.

Cites

No record.

References

No record.

Cite

APA: Copy

KARIMI, A., & SALEHI, R.. (2005). IN VITRO INTERACTION AND COLOCALIZATION OF HSV-1 ORF P WITH A CELLULAR SPLICING FACTOR (SC35) USING PULLDOWN ASSAY. IRANIAN BIOMEDICAL JOURNAL, 9(2), 67-71. SID. https://sid.ir/paper/277017/en

Vancouver: Copy

KARIMI A., SALEHI R.. IN VITRO INTERACTION AND COLOCALIZATION OF HSV-1 ORF P WITH A CELLULAR SPLICING FACTOR (SC35) USING PULLDOWN ASSAY. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2005;9(2):67-71. Available from: https://sid.ir/paper/277017/en

IEEE: Copy

A. KARIMI, and R. SALEHI, “IN VITRO INTERACTION AND COLOCALIZATION OF HSV-1 ORF P WITH A CELLULAR SPLICING FACTOR (SC35) USING PULLDOWN ASSAY,” IRANIAN BIOMEDICAL JOURNAL, vol. 9, no. 2, pp. 67–71, 2005, [Online]. Available: https://sid.ir/paper/277017/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops