AFFINITY PURIFICATION AND CHARACTERIZATION OF RECOMBINANT BACILLUS SPHAERICUS HENYLALANINE DEHYDROGENASE PRODUCED BY PET EXPRESSION VECTOR SYSTEM

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OMIDINIA E.; RASHIDPOURAIE R.; TAHERKHANI H.; ASANO Y.; KHATHAMI SH.; OMUMI A.R.; MIRZAHOSSEINI H.; SAMADI A.; GHADIRI A.; VAN DER LELIE D.

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Journal Paper

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Journal: Iranian Biomedical Journal Year:2002 | Volume:6 | Issue:1 Page(s): 31-36

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Title

AFFINITY PURIFICATION AND CHARACTERIZATION OF RECOMBINANT BACILLUS SPHAERICUS HENYLALANINE DEHYDROGENASE PRODUCED BY PET EXPRESSION VECTOR SYSTEM

Author(s)

Keywords

BACILLUS SPHAERICUS

L-PHENYLALANINE DEHYDROGENASE (PHEDH)

AFFINITY PURIFICATION

EXPRESSION

Abstract

Cloning and EXPRESSION of the L-phenylalanine dehydrogenase gene, from B. sphaericus in E. coli were done. The gene was cloned in the vector pET16b and transformed into E. coli BL21 (DE3). The functional form of the L-phenylalanine dehydrogenase enzyme was purified by AFFINITY PURIFICATION techniques, taking advantage of the ability of this enzyme to bind to the nucleotide site affinity dye, Reactive Blue 4. Approximately 3 mg of highly purified recombinant enzyme was obtained from 950 mg cell pellet (wet weight). The Relative molecular mass of the L-phenylalanine subunits was about 41 kDa by 10% SDS-PAGE. Using this method, the enzyme was obtained with a yield of 28%, and had a specific activity of 577.3 U/mg protein, which is purified 88 times. This method was provided a facile and effective way for preparing the enzyme with a good yield that suitable for analytical purposes

Cites

DEVELOPMENT AND APPLICATION OF AQUEOUS TWO-PHASE PARTITION FOR THE RECOVERY AND SEPARATION OF RECOMBINANT PHENYLALANINE DEHYDROGENASE

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APA: Copy

OMIDINIA, E., RASHIDPOURAIE, R., TAHERKHANI, H., ASANO, Y., KHATHAMI, SH., OMUMI, A.R., MIRZAHOSSEINI, H., SAMADI, A., GHADIRI, A., & VAN DER LELIE, D.. (2002). AFFINITY PURIFICATION AND CHARACTERIZATION OF RECOMBINANT BACILLUS SPHAERICUS HENYLALANINE DEHYDROGENASE PRODUCED BY PET EXPRESSION VECTOR SYSTEM. IRANIAN BIOMEDICAL JOURNAL, 6(1), 31-36. SID. https://sid.ir/paper/277053/en

Vancouver: Copy

OMIDINIA E., RASHIDPOURAIE R., TAHERKHANI H., ASANO Y., KHATHAMI SH., OMUMI A.R., MIRZAHOSSEINI H., SAMADI A., GHADIRI A., VAN DER LELIE D.. AFFINITY PURIFICATION AND CHARACTERIZATION OF RECOMBINANT BACILLUS SPHAERICUS HENYLALANINE DEHYDROGENASE PRODUCED BY PET EXPRESSION VECTOR SYSTEM. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2002;6(1):31-36. Available from: https://sid.ir/paper/277053/en

IEEE: Copy

E. OMIDINIA, R. RASHIDPOURAIE, H. TAHERKHANI, Y. ASANO, SH. KHATHAMI, A.R. OMUMI, H. MIRZAHOSSEINI, A. SAMADI, A. GHADIRI, and D. VAN DER LELIE, “AFFINITY PURIFICATION AND CHARACTERIZATION OF RECOMBINANT BACILLUS SPHAERICUS HENYLALANINE DEHYDROGENASE PRODUCED BY PET EXPRESSION VECTOR SYSTEM,” IRANIAN BIOMEDICAL JOURNAL, vol. 6, no. 1, pp. 31–36, 2002, [Online]. Available: https://sid.ir/paper/277053/en

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