PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST BRUCELLA ABORTUS S (99) SURFACE ANTIGENS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

FARSHAD SH.; TAVAKKOLI A.; MEHRABANPOUR M.J.; NAM AVARI M.M.; HOSSEINI S.M.H.; GHADERI A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Biomedical Journal Year:2002 | Volume:6 | Issue:1 Page(s): 7-12

Download Full-Text

View:

352

Download:

150

Cites:

Information Journal Paper

Title

PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST BRUCELLA ABORTUS S (99) SURFACE ANTIGENS

Author(s)

Keywords

BRUCELLA

ANTIGENS

MONOCLONAL ANTIBODY

Abstract

By immunizing mice with killed whole bacterial cells of BRUCELLA abortus S (99), a panel of six hybridomas producing monoclonal antibodies (mAb) specific for the surface ANTIGENS of this bacterium were produced. ELISA was used to screen the hybridoma supernatants. Immunoblots of the cell extract indicated that three mAb were specific for S-LPS (Ba-1, Ba-2, Ba-3) and three others were reactive with major outer membrane proteins (OMP) (Ba-4, Ba-5, Ba-6). The OMP recognized by these antibodies were the proteins with molecular masses of 25-27 kDa (Ba-4, Ba-5) and 36-38 kDa (Ba-6). None of the four mAb including Ba-3, Ba-4, Ba-5 and Ba-6 cross reacted with any other bacteria close to BRUCELLA abortus, but Ba-1 and Ba-2 cross reacted with B. melitensis 16M and B. suis. By using cell extract and killed whole cell Ag in ELISA, it was indicated that all mAb except Ba-6 have better reactivity with cell extract Ag, but Ba-6 mAb reacted with killed whole cell Ag better than cell extract Ag

Cites

No record.

References

No record.

Cite

APA: Copy

FARSHAD, SH., TAVAKKOLI, A., MEHRABANPOUR, M.J., NAM AVARI, M.M., HOSSEINI, S.M.H., & GHADERI, A.. (2002). PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST BRUCELLA ABORTUS S (99) SURFACE ANTIGENS . IRANIAN BIOMEDICAL JOURNAL, 6(1), 7-12. SID. https://sid.ir/paper/277054/en

Vancouver: Copy

FARSHAD SH., TAVAKKOLI A., MEHRABANPOUR M.J., NAM AVARI M.M., HOSSEINI S.M.H., GHADERI A.. PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST BRUCELLA ABORTUS S (99) SURFACE ANTIGENS . IRANIAN BIOMEDICAL JOURNAL[Internet]. 2002;6(1):7-12. Available from: https://sid.ir/paper/277054/en

IEEE: Copy

SH. FARSHAD, A. TAVAKKOLI, M.J. MEHRABANPOUR, M.M. NAM AVARI, S.M.H. HOSSEINI, and A. GHADERI, “PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST BRUCELLA ABORTUS S (99) SURFACE ANTIGENS ,” IRANIAN BIOMEDICAL JOURNAL, vol. 6, no. 1, pp. 7–12, 2002, [Online]. Available: https://sid.ir/paper/277054/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops