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Information Journal Paper

Title

REPLICATION CHARACTERISTICS OF HERPES SIMPLEX VIRUS TYPE-1 (HSV-1) RECOMBINANTS IN 3 TYPES OF TISSUE CULTURES

Pages

  95-101

Keywords

HERPES SIMPLEX VIRUS TYPE-1 (HSV-1) RECOMBINANTS 
OPEN READING FRAME P (ORF P) 

Abstract

 A complication in the analysis of the role of ICP34.5 gene in the herpes simplex virus type-1 (HSV-1) lifecycle is the presence of overlapping antisense gene, open reading frame P (ORF P), which is also deleted in HSV-1 ICP34.5 negative mutants. A HSV-1 wild type strain (17+) ICP34.5/ORF P deletion mutant (1716) is totally avirulent in animal models and impaired in a number of in vitro functions: replication in 3T6 cells; and replication and shutoff of cellular protein synthesis in SK-N-SH cells. To attribute characteristics of 1716 to each of these two genes (ICP34.5 or ORF P), a number of HSV-1 recombinant viruses that express ICP34.5 and ORF P independently were constructed, purified and characterized. The parent of these recombinants is 1716 and they are (so called): 1622, expressing ICP34.5; 1624/24.5, expressing ORF P; and 1625, expressing both ICP34.5 and ORF P in separate loci. Using homologous recombination in cell culture, the recombinants were constructed and their DNA were analyzed by Southern-blotting. Expression of ICP34.5 and ORF P from the recombinants was checked by Western-blotting. Using cell culture, titration and plaque assay techniques, replication kinetics of the recombinants were compared with 17+ and 1716 in 3 cell lines, BHK, 3T6, and SK-N-SH. The results showed that (i) ICP34.5 restored the ability to replicate and prevent host shutoff similar to wild type in SK-N-SH cells; (ii) ICP34.5 restored the replication phenotype to near wild type levels in 3T6 cells; and (iii) ORF P expression had no effect on the replication of these mutants. The characteristics of 1716 is obviously due to the lack of ICP34.5 and ORF P has no role in the characteristics studied. Thus, the function of ORF P still has to be determined.

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    • Cite

    APA: Copy

    KARIMI, A., & MACLEAN, A.. (2005). REPLICATION CHARACTERISTICS OF HERPES SIMPLEX VIRUS TYPE-1 (HSV-1) RECOMBINANTS IN 3 TYPES OF TISSUE CULTURES. IRANIAN BIOMEDICAL JOURNAL, 9(3), 95-101. SID. https://sid.ir/paper/277076/en

    Vancouver: Copy

    KARIMI A., MACLEAN A.. REPLICATION CHARACTERISTICS OF HERPES SIMPLEX VIRUS TYPE-1 (HSV-1) RECOMBINANTS IN 3 TYPES OF TISSUE CULTURES. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2005;9(3):95-101. Available from: https://sid.ir/paper/277076/en

    IEEE: Copy

    A. KARIMI, and A. MACLEAN, “REPLICATION CHARACTERISTICS OF HERPES SIMPLEX VIRUS TYPE-1 (HSV-1) RECOMBINANTS IN 3 TYPES OF TISSUE CULTURES,” IRANIAN BIOMEDICAL JOURNAL, vol. 9, no. 3, pp. 95–101, 2005, [Online]. Available: https://sid.ir/paper/277076/en

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