COMPARISON OF PCR AND CULTURE METHODS FOR DIAGNOSIS OF ENTEROPATHOGENIC CAMPYLOBACTER IN FOWL FECES

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HAVAEI S.A.; FAZELI S.A.; SALEHI R.; BOKAEIAN M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Biomedical Journal Year:2006 | Volume:10 | Issue:1 Page(s): 47-50

Download Full-Text

View:

354

Download:

144

Cites:

Information Journal Paper

Title

COMPARISON OF PCR AND CULTURE METHODS FOR DIAGNOSIS OF ENTEROPATHOGENIC CAMPYLOBACTER IN FOWL FECES

Author(s)

HAVAEI S.A. | FAZELI S.A. | SALEHI R. | BOKAEIAN M. | Issue Writer Certificate

Keywords

CAMPYLOBACTER

CULTURE

FOWL

PCR

DIAGNOSIS

Abstract

Enteritis due to CAMPYLOBACTER is the most common cause of acute bacterial diarrhea worldwide. In most cases, infection occurs as a result of consuming contaminated water or food, especially raw meat of FOWLs. CAMPYLOBACTERs are saccharolytic and fastidious bacteria. These traits limit the number of available biochemical tests by which isolates may be differentiated. These limitations might, in principle, be overcome by the use of PCR techniques, which is the aim of the present study. To compare the CULTURE technique with PCR assay, a total of 116 fecal samples from FOWLs were tested using these two techniques for the presence of CAMPYLOBACTERs. CAMPYLOBACTER strains were isolated from 11 (9.4%) out of 116 fecal CULTUREs from FOWLs (8 C. jejuni and 3 C. coli). Using PCR assays, the number of positive CAMPYLOBACTERs increased to 27 (23%). Of these 27 positive samples, 18 were C. jejuni and 9 were C. coli. The sensitivity and specificity of PCR in comparison to the CULTURE method were found to be 100 and 84.7%, respectively. According to the present study, it is proposed that the PCR is a reliable and sensitive method which can be used as a diagnostic technique for the detection of CAMPYLOBACTER in FOWLs’ samples.

Cites

No record.

References

No record.

Cite

APA: Copy

HAVAEI, S.A., FAZELI, S.A., SALEHI, R., & BOKAEIAN, M.. (2006). COMPARISON OF PCR AND CULTURE METHODS FOR DIAGNOSIS OF ENTEROPATHOGENIC CAMPYLOBACTER IN FOWL FECES. IRANIAN BIOMEDICAL JOURNAL, 10(1), 47-50. SID. https://sid.ir/paper/277090/en

Vancouver: Copy

HAVAEI S.A., FAZELI S.A., SALEHI R., BOKAEIAN M.. COMPARISON OF PCR AND CULTURE METHODS FOR DIAGNOSIS OF ENTEROPATHOGENIC CAMPYLOBACTER IN FOWL FECES. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2006;10(1):47-50. Available from: https://sid.ir/paper/277090/en

IEEE: Copy

S.A. HAVAEI, S.A. FAZELI, R. SALEHI, and M. BOKAEIAN, “COMPARISON OF PCR AND CULTURE METHODS FOR DIAGNOSIS OF ENTEROPATHOGENIC CAMPYLOBACTER IN FOWL FECES,” IRANIAN BIOMEDICAL JOURNAL, vol. 10, no. 1, pp. 47–50, 2006, [Online]. Available: https://sid.ir/paper/277090/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops