A STUDY OF THE OXIDATION-INDUCED CONFORMATIONAL AND FUNCTIONAL CHANGES IN NEUROSERPIN

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MOHSENIFAR A.; LOTFI A.S.; ALAMEH A.A.; ETEMADIKIA B.; HASANNIA S.; RANJBAR B.; HASANI L.; ZAKER F.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Biomedical Journal Year:2007 | Volume:11 | Issue:1 Page(s): 41-46

Download Full-Text

View:

378

Download:

382

Cites:

Information Journal Paper

Title

A STUDY OF THE OXIDATION-INDUCED CONFORMATIONAL AND FUNCTIONAL CHANGES IN NEUROSERPIN

Author(s)

Keywords

NEUROSERPIN

REACTIVE OXYGEN SPECIES

CONFORMATIONAL DISORDER

SURFACE HYDROPHOBICITY

INHIBITORY ACTIVITY

Abstract

Background: NEUROSERPIN, a member of the Serine Proteinase Inhibitor (Serpin) super family, is known to be a neuroprotective factor in the focal ischemic stroke followed by reducing the microglial activation. NEUROSERPIN is a protein rich of methionine residues that can scavenge the free radical species which may increase its neuroprotective effect. On the other hand, the oxidative modifications of the amino acid residues in NEUROSERPIN may lead to changes in its conformation and function. In this study, it was investigated the changes in the conformation and the function of the oxidized NEUROSERPIN. Methods: NEUROSERPIN expressed in E. coli, BL21 or M15 harboring plasmid pQE81L containing NEUROSERPIN cDNA. Expressed NEUROSERPIN was purified by resin sulfopropyl A50 precharged with 0.1 M NiSO4 under denaturing condition. NEUROSERPIN was oxidized under oxidative stress condition in the presence of different concentration of hydrogen peroxide. The oxidation of NEUROSERPIN was conveniently detected by a carbonyl content assay using 2, 4 dinitrophenylhydrazine. Changes in tertiary structure of NEUROSERPIN were monitored by spectrofluorimeter to study the alteration of intrinsic fluorescence and also fluorescence of 8-anilinonaphthalin-1 sulfonic acid (ANS) in native and oxidized form of NEUROSERPIN. Results: Total expressed NEUROSERPIN was estimated 4-5 mg/lit in 2XYT culture media. SDS-PAGE analysis of purified NEUROSERPIN showed a single band which reflects the efficiency of the resin SP A50 for purification of the proteins containing 6×His tag. Carbonyl content of oxidized and native NEUROSERPIN was estimated 12.3 ± 0.3 and 0.45 ± 0.05, respectively. The INHIBITORY ACTIVITY of oxidized NEUROSERPIN decreased up to 40-60% as compared with native form of NEUROSERPIN. Intrinsic fluorescence and also the emission of ANS bind to the hydrophobic region of the protein altered from 380 to 85 and in the case of ANS from 105 to 150 in oxidized and native form of NEUROSERPIN, respectively. Conclusion: The decreased intrinsic fluorescence intensity, an enhancement in the fluorescence of ANS, and loss of the INHIBITORY ACTIVITY up to 40-60% in NEUROSERPIN, all suggested a conformational modification in the protein under the oxidative stress condition. Remaining the INHIBITORY ACTIVITY of NEUROSERPIN reflects that the protein tolerates the oxidative stress condition effectively.

Cites

No record.

References

No record.

Cite

APA: Copy

MOHSENIFAR, A., LOTFI, A.S., ALAMEH, A.A., ETEMADIKIA, B., HASANNIA, S., RANJBAR, B., HASANI, L., & ZAKER, F.. (2007). A STUDY OF THE OXIDATION-INDUCED CONFORMATIONAL AND FUNCTIONAL CHANGES IN NEUROSERPIN. IRANIAN BIOMEDICAL JOURNAL, 11(1), 41-46. SID. https://sid.ir/paper/277132/en

Vancouver: Copy

MOHSENIFAR A., LOTFI A.S., ALAMEH A.A., ETEMADIKIA B., HASANNIA S., RANJBAR B., HASANI L., ZAKER F.. A STUDY OF THE OXIDATION-INDUCED CONFORMATIONAL AND FUNCTIONAL CHANGES IN NEUROSERPIN. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2007;11(1):41-46. Available from: https://sid.ir/paper/277132/en

IEEE: Copy

A. MOHSENIFAR, A.S. LOTFI, A.A. ALAMEH, B. ETEMADIKIA, S. HASANNIA, B. RANJBAR, L. HASANI, and F. ZAKER, “A STUDY OF THE OXIDATION-INDUCED CONFORMATIONAL AND FUNCTIONAL CHANGES IN NEUROSERPIN,” IRANIAN BIOMEDICAL JOURNAL, vol. 11, no. 1, pp. 41–46, 2007, [Online]. Available: https://sid.ir/paper/277132/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops