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Title

INDUCTION OF APOPTOSIS ON K562 CELL LINE AND DOUBLE STRAND BREAKS ON COLON CANCER CELL LINE EXPRESSING HIGH AFFINITY RECEPTOR FOR GRANULOCYTE MACROPHAGE-COLONY STIMULATING FACTOR (GM-CSF)

Pages

  1-6

Keywords

SHIGA TOXIN GRANULOCYTE MACROPHAGE-COLONY STIMULATING FACTOR (STXA1- GM- CSF) 
DOUBLE STRAND BREAKS (DSB) 

Abstract

 Background: Immunotoxins are comprised of both the cell targeting and the cell killing moieties. We previously established a new immunotoxin, i.e. Shiga toxin granulocyte macrophage- colony stimulating factor (StxA1- GM- CSF), comprises of catalytic domain of Stx, as a killing moiety and GM-CSF, as a cell targeting moiety. In this study, the ability of the immunotoxin to induce APOPTOSIS and double strand breaks (DSB) on different cell lines was investigated.Methods: The recombinant hybrid protein was expressed in bacterial expression system and purified with nickel- nitrilotriacetate acid resin. The K562 (erythroid leukemia) cell line and LS174 (colon carcinoma) were used in this study. The neutral comet assay was carried out for the detection of DSB and Hoechst staining was performed for APOPTOSIS.Results: StxA1- GM- CSF effectively induced APOPTOSIS on K562 cell line and DNA Double Strand Break (DSB) were observed on colon cancer cell line treated with StxA1- GM- CSF.Conclusion: This novel action i.e. DNA damage might be a relevant mechanism of action for StxA1- GM- CSF that is designed to act as immunotoxin, although further investigation is required.

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    APA: Copy

    HABIBI ROUDKENAR, M., BOUZARI, S., OLOUMI, MANA, KUWAHARA, Y., BABA, T., FUKUMOTO, M., & MOHAMMADI ROUSHANDEH, A.. (2008). INDUCTION OF APOPTOSIS ON K562 CELL LINE AND DOUBLE STRAND BREAKS ON COLON CANCER CELL LINE EXPRESSING HIGH AFFINITY RECEPTOR FOR GRANULOCYTE MACROPHAGE-COLONY STIMULATING FACTOR (GM-CSF). IRANIAN BIOMEDICAL JOURNAL, 12(1), 1-6. SID. https://sid.ir/paper/277176/en

    Vancouver: Copy

    HABIBI ROUDKENAR M., BOUZARI S., OLOUMI MANA, KUWAHARA Y., BABA T., FUKUMOTO M., MOHAMMADI ROUSHANDEH A.. INDUCTION OF APOPTOSIS ON K562 CELL LINE AND DOUBLE STRAND BREAKS ON COLON CANCER CELL LINE EXPRESSING HIGH AFFINITY RECEPTOR FOR GRANULOCYTE MACROPHAGE-COLONY STIMULATING FACTOR (GM-CSF). IRANIAN BIOMEDICAL JOURNAL[Internet]. 2008;12(1):1-6. Available from: https://sid.ir/paper/277176/en

    IEEE: Copy

    M. HABIBI ROUDKENAR, S. BOUZARI, MANA OLOUMI, Y. KUWAHARA, T. BABA, M. FUKUMOTO, and A. MOHAMMADI ROUSHANDEH, “INDUCTION OF APOPTOSIS ON K562 CELL LINE AND DOUBLE STRAND BREAKS ON COLON CANCER CELL LINE EXPRESSING HIGH AFFINITY RECEPTOR FOR GRANULOCYTE MACROPHAGE-COLONY STIMULATING FACTOR (GM-CSF),” IRANIAN BIOMEDICAL JOURNAL, vol. 12, no. 1, pp. 1–6, 2008, [Online]. Available: https://sid.ir/paper/277176/en

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