RAPID SCREENING OF TOXIGENIC VIBRIO CHOLERAE O1 STRAINS FROM SOUTH IRAN BY PCR-ELISA

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MOUSAVI S.L.; NAZARIAN SH.; AMANI J.; KARIMI RAHJERDI A.

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Journal Paper

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Journal: Iranian Biomedical Journal Year:2008 | Volume:12 | Issue:1 Page(s): 15-21

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Title

RAPID SCREENING OF TOXIGENIC VIBRIO CHOLERAE O1 STRAINS FROM SOUTH IRAN BY PCR-ELISA

Author(s)

MOUSAVI S.L. | NAZARIAN SH. | AMANI J. | KARIMI RAHJERDI A. | Issue Writer Certificate

Keywords

VIBRIO CHOLERA

PCR- ELISA

IMMUNOSORBENT ASSAY

Abstract

Background: The ability to sensitively detect VIBRIO CHOLERA with PCR-ELISA method represents a considerable advancement over alternative more time-consuming methods for detection of this pathogen. The aim of this research is to evaluate the suitability of a PCR-enzyme-linked IMMUNOSORBENT ASSAY for sensitive and rapid detection of V. cholera O1. Methods: The 398-bp sequence of a gene that codes for the cholera toxin B subunit was amplified by PCR. The digoxigenin-labeled amplified products were coated on microplates and detected by ELISA. The PCR product was also hybridized with biotin labelled probe and detected by ELISA using streptavidin. Results and Conclusion: The specificity of the PCR was determined using 10 bacterial strains and 50 samples from south Iran. The detection limit was 0.5 pg of the genomic DNA and five bacterial cells. Adaptation of PCR into PCR-ELISA assay format facilitates specific and sensitive detection and diagnosis of human cholera disease. We conclude that this PCR-ELISA is a diagnostic method that specifically detects toxin genes in V. cholera O1 strains. It is more rapid and less cumbersome than other diagnostic methods for detection of toxicity in these strains.

Cites

DETECTION OF HEAT-LABILE TOXIN IN ENTEROTOXIGENIC ESCHERICHIA COLI USING PCR-ELISA TECHNIQUE

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APA: Copy

MOUSAVI, S.L., NAZARIAN, SH., AMANI, J., & KARIMI RAHJERDI, A.. (2008). RAPID SCREENING OF TOXIGENIC VIBRIO CHOLERAE O1 STRAINS FROM SOUTH IRAN BY PCR-ELISA. IRANIAN BIOMEDICAL JOURNAL, 12(1), 15-21. SID. https://sid.ir/paper/277180/en

Vancouver: Copy

MOUSAVI S.L., NAZARIAN SH., AMANI J., KARIMI RAHJERDI A.. RAPID SCREENING OF TOXIGENIC VIBRIO CHOLERAE O1 STRAINS FROM SOUTH IRAN BY PCR-ELISA. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2008;12(1):15-21. Available from: https://sid.ir/paper/277180/en

IEEE: Copy

S.L. MOUSAVI, SH. NAZARIAN, J. AMANI, and A. KARIMI RAHJERDI, “RAPID SCREENING OF TOXIGENIC VIBRIO CHOLERAE O1 STRAINS FROM SOUTH IRAN BY PCR-ELISA,” IRANIAN BIOMEDICAL JOURNAL, vol. 12, no. 1, pp. 15–21, 2008, [Online]. Available: https://sid.ir/paper/277180/en

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