مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

THE ENHANCEMENT OF BIODESULFURIZATION ACTIVITY IN A NOVELINDIGENOUS ENGINEERED PSEUDOMONAS PUTIDA

Pages

  207-213

Abstract

 Background: The combustion of sulfur-rich fossil fuels leads to release of sulfur oxide pollution in the environment. In BIODESULFURIZATION process, an organism is able to remove sulfur from fossil fuels without decreasing the caloric value of those substrates. The main aim of this research was to design a recombinant microorganism to remove the highest amount of sulfur compounds in fossil fuels.Methods: Three genes (dszA, B, C) from dsz operon are responsible for the 4S pathway (biodesulfurization pathway) in Rhodococcus erythropolisIGTS8 were inserted into the chromosome of a novel indigenous Pseudomonas putida. The reaction catalyzed by products of dszA, B, C genes require FMNH2 supplied by dszD enzyme. Thus, pVLT 31 vector harboringdszD gene was transferred into this recombinant strain.Results: The results demonstrated a higher BIODESULFURIZATION activity when the flavin reductase gene was transferred into recombinant P. putida harboringdsz A, B, C. These results were approved by the Gibbs test and HPLC analysis.Conclusion: These analyses showed that this novel indigenous engineeredP. putida could be a promising candidate for an industrial and environmental application for BIODESULFURIZATION process.

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  • Cite

    APA: Copy

    RAHEB, JAMSHID, HAJIPOUR, MOHAMMAD JAVAD, SAADATI, MOJTABA, RASEKH, BEHNAM, & MEMARI, BABAK. (2009). THE ENHANCEMENT OF BIODESULFURIZATION ACTIVITY IN A NOVELINDIGENOUS ENGINEERED PSEUDOMONAS PUTIDA. IRANIAN BIOMEDICAL JOURNAL, 13(4), 207-213. SID. https://sid.ir/paper/277245/en

    Vancouver: Copy

    RAHEB JAMSHID, HAJIPOUR MOHAMMAD JAVAD, SAADATI MOJTABA, RASEKH BEHNAM, MEMARI BABAK. THE ENHANCEMENT OF BIODESULFURIZATION ACTIVITY IN A NOVELINDIGENOUS ENGINEERED PSEUDOMONAS PUTIDA. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2009;13(4):207-213. Available from: https://sid.ir/paper/277245/en

    IEEE: Copy

    JAMSHID RAHEB, MOHAMMAD JAVAD HAJIPOUR, MOJTABA SAADATI, BEHNAM RASEKH, and BABAK MEMARI, “THE ENHANCEMENT OF BIODESULFURIZATION ACTIVITY IN A NOVELINDIGENOUS ENGINEERED PSEUDOMONAS PUTIDA,” IRANIAN BIOMEDICAL JOURNAL, vol. 13, no. 4, pp. 207–213, 2009, [Online]. Available: https://sid.ir/paper/277245/en

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