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1

Information Journal Paper

Title

DEGRADATION OF EXTRACELLULAR MATRIX MOLECULES IN INTERLEUKIN-1α TREATED BOVINE NASAL CARTILAGE

Pages

  158-163

Keywords

CARTILAGE OLIGOMERIC MATRIX PROTEIN (COMP) 
FIBROMODULIN (FM) 

Abstract

 Background: This work aimed to show and compare the degradation time of some of cartilage extracellular matrix components using an invitro model for cartilage degradation induced by interleukin-1a. It is known that elucidation of molecular events under Interleukin-1a induction of bovine nasal cartilage could obtain useful data to understand more about involving mechanisms for tissue breakdown in joint disease.Methods: The cartilage was taken from an adult bovine in a local slaughterhouse. After removing the whole perichondrium, the equal 2 mm diameter pieces of bovine nasal cartilage were punched out and cultured in Dulbecco's modified Eagle's medium DMEM with or without 10 ng/ml Interleukin-1a for 24 days. Each 3 days, the media were removed and exchanged by fresh media, and the removed media were stored in -20°C.Sodium dodecyl sulphate/polyacrylamid-gel electrophoresis SDS-PAGE and Western-blot methods were used for analyzing the samples.Results: The first fragment of fibromodulin (FM) was seen at day 6 and further fragments were appeared at day 18. Cartilage oligomeric matrix protein (COMP) releasing was as a successive pattern during culture period and the first fragment was found at day 6. COLLAGEN IX fragments were seen at day 9 and in a progressive pattern until the end of the study.Conclusion: This study shows that FM and COMP could be considered as the suitable candidates for studying the mechanisms that participate in the cartilage degradations.

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    APA: Copy

    SHOHANI, BEHNAZ, ORAZIZADEH, MAHMOUD, HASHEMITABAR, MAHMOUD, & HEINEGARD, DICK. (2010). DEGRADATION OF EXTRACELLULAR MATRIX MOLECULES IN INTERLEUKIN-1α TREATED BOVINE NASAL CARTILAGE. IRANIAN BIOMEDICAL JOURNAL, 14(4), 158-163. SID. https://sid.ir/paper/277252/en

    Vancouver: Copy

    SHOHANI BEHNAZ, ORAZIZADEH MAHMOUD, HASHEMITABAR MAHMOUD, HEINEGARD DICK. DEGRADATION OF EXTRACELLULAR MATRIX MOLECULES IN INTERLEUKIN-1α TREATED BOVINE NASAL CARTILAGE. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2010;14(4):158-163. Available from: https://sid.ir/paper/277252/en

    IEEE: Copy

    BEHNAZ SHOHANI, MAHMOUD ORAZIZADEH, MAHMOUD HASHEMITABAR, and DICK HEINEGARD, “DEGRADATION OF EXTRACELLULAR MATRIX MOLECULES IN INTERLEUKIN-1α TREATED BOVINE NASAL CARTILAGE,” IRANIAN BIOMEDICAL JOURNAL, vol. 14, no. 4, pp. 158–163, 2010, [Online]. Available: https://sid.ir/paper/277252/en

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