مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

TRANS-DIFFERENTIATION OF THE ADIPOSE TISSUE-DERIVED STEM CELLS INTO NEURON-LIKE CELLS EXPRESSING NEUROTROPHINS BY SELEGILINE

Pages

  113-121

Abstract

 Background: Adult stem cells (ASC) are undifferentiated cells found throughout the body. These cells are promising tools for cell replacement therapy in neurodegenerative disease. Adipose tissue is the most abundant and accessible source of ASC. This study was conducted to evaluate effect of SELEGILINE on differentiation of adiposederived stem cells (ADSC) into functional neuron-like cells (NLC), and also level of the NEUROTROPHIN expression in differentiated cells.Methods: ADSC were transdifferentiated into NLC using SELEGILINE where CD90, CD49d, CD31, CD106 and CD45 were used as markers for ADSC identification. Lipogenic and osteogenic differentiation of ADSC were used to characterize the ADSC. ADSC were treated with SELEGILINE at different concentrations (from 10-6 to 10-11 mM) and time points (3, 6, 12, 24 and 48 h). Percentage of viable cells, nestin and neurofilament 68 (NF-68) immunoreactive cells were used as markers for differentiation. The optimal dose for NEUROTROPHIN expressions in differentiating cells was evaluated using reverse transcriptase-PCR. NLC function was evaluated by loading and unloading with FM1-43 dye.Results: ADSC were immunoreactive to CD90 (95.67 ± 2.26), CD49d (71.52 ± 6.64) and CD31 (0.6 ± 0.86), but no immunoreactivity was detected for CD106 and CD45. The results of neural differentiation showed the highest percentage of nestin and NF-68 positive cells at 10-9 mM concentration of SELEGILINE (exposed for 24 h). The differentiated cells expressed synapsin and NEUROTROPHIN genes except brainderived neurotrophic factor.Conclusion: ADSC can be an alternative source in cell-based therapy for neurodegenerative diseases using SELEGILINE to induce ADSC differentiation to neuronal lineage.

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    APA: Copy

    ABDANIPOUR, ALIREZA, TIRAIHI, TAKI, & DELSHAD, ALIREZA. (2011). TRANS-DIFFERENTIATION OF THE ADIPOSE TISSUE-DERIVED STEM CELLS INTO NEURON-LIKE CELLS EXPRESSING NEUROTROPHINS BY SELEGILINE. IRANIAN BIOMEDICAL JOURNAL, 15(4), 113-121. SID. https://sid.ir/paper/277274/en

    Vancouver: Copy

    ABDANIPOUR ALIREZA, TIRAIHI TAKI, DELSHAD ALIREZA. TRANS-DIFFERENTIATION OF THE ADIPOSE TISSUE-DERIVED STEM CELLS INTO NEURON-LIKE CELLS EXPRESSING NEUROTROPHINS BY SELEGILINE. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2011;15(4):113-121. Available from: https://sid.ir/paper/277274/en

    IEEE: Copy

    ALIREZA ABDANIPOUR, TAKI TIRAIHI, and ALIREZA DELSHAD, “TRANS-DIFFERENTIATION OF THE ADIPOSE TISSUE-DERIVED STEM CELLS INTO NEURON-LIKE CELLS EXPRESSING NEUROTROPHINS BY SELEGILINE,” IRANIAN BIOMEDICAL JOURNAL, vol. 15, no. 4, pp. 113–121, 2011, [Online]. Available: https://sid.ir/paper/277274/en

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