AN ENTEROVIRUS-LIKE RNA CONSTRUCT FOR COLON CANCER SUICIDE GENE THERAPY

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

RASEKHIAN MAHSA; TEIMOORI TOOLABI LADAN; AMINI SAFIEH; AZADMANESH KAYHAN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Biomedical Journal Year:2015 | Volume:19 | Issue:3 Page(s): 124-132

Download Full-Text

View:

358

Download:

203

Cites:

Information Journal Paper

Title

AN ENTEROVIRUS-LIKE RNA CONSTRUCT FOR COLON CANCER SUICIDE GENE THERAPY

Author(s)

RASEKHIAN MAHSA | TEIMOORI TOOLABI LADAN | AMINI SAFIEH | AZADMANESH KAYHAN | Issue Writer Certificate

Keywords

THYMIDINE KINASE

POLIO 3'NCR

MIR-143

Abstract

Background: In gene therapy, the use of RNA molecules as therapeutic agents has shown advantages over plasmid DNA, including higher levels of safety. However, transient nature of RNA has been a major obstacle in application of RNA in gene therapy.Methods: Here, we used the internal ribosomal entry site of encephalomyocarditis virus and the 3’ non-translated region of Poliovirus to design an enterovirus-like RNA for the expression of a reporter gene (enhanced green fluorescent protein) and a suicide gene (THYMIDINE KINASE of herpes simplex virus). The expression of these genes was evaluated by flow cytometry and cytotoxicity assay in human colorectal adenocarcinoma cell line (SW480). We then armed RNA molecules with a target sequence for hsa-miR-143 to regulate their expression by microRNA (miRNA) mimics.Results: The results showed effective expression of both genes by Entrovirus-like RNA constructs. The data also showed that the restoration of hsa-miR-143 expression in SW480 leads to a significant translation repression of the introduced reporter and suicide genes.Conclusion: Collectively, our data suggest the potential use of Entrovirus-like RNA molecules in suicide gene therapy.Additionally, as a consequence of the possible downregulated miRNA expression in cancerous tissues, a decreased expression of gene therapy constructs armed with target sequences for such miRNA in cancer tissue is expected.

Cites

No record.

References

No record.

Cite

APA: Copy

RASEKHIAN, MAHSA, TEIMOORI TOOLABI, LADAN, AMINI, SAFIEH, & AZADMANESH, KAYHAN. (2015). AN ENTEROVIRUS-LIKE RNA CONSTRUCT FOR COLON CANCER SUICIDE GENE THERAPY. IRANIAN BIOMEDICAL JOURNAL, 19(3), 124-132. SID. https://sid.ir/paper/277339/en

Vancouver: Copy

RASEKHIAN MAHSA, TEIMOORI TOOLABI LADAN, AMINI SAFIEH, AZADMANESH KAYHAN. AN ENTEROVIRUS-LIKE RNA CONSTRUCT FOR COLON CANCER SUICIDE GENE THERAPY. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2015;19(3):124-132. Available from: https://sid.ir/paper/277339/en

IEEE: Copy

MAHSA RASEKHIAN, LADAN TEIMOORI TOOLABI, SAFIEH AMINI, and KAYHAN AZADMANESH, “AN ENTEROVIRUS-LIKE RNA CONSTRUCT FOR COLON CANCER SUICIDE GENE THERAPY,” IRANIAN BIOMEDICAL JOURNAL, vol. 19, no. 3, pp. 124–132, 2015, [Online]. Available: https://sid.ir/paper/277339/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops