IN VITRO LABELING OF NEURAL STEM CELLS WITH POLY-L-LYSINE COATED SUPER PARAMAGNETIC NANOPARTICLES FOR GREEN FLUORESCENT PROTEIN TRANSFECTION

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ALBUKHATY SALIM; NADERI MANESH HOSSEIN; TIRAIHI TAKI

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Journal: Iranian Biomedical Journal Year:2013 | Volume:17 | Issue:2 Page(s): 71-76

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Title

IN VITRO LABELING OF NEURAL STEM CELLS WITH POLY-L-LYSINE COATED SUPER PARAMAGNETIC NANOPARTICLES FOR GREEN FLUORESCENT PROTEIN TRANSFECTION

Author(s)

ALBUKHATY SALIM | NADERI MANESH HOSSEIN | TIRAIHI TAKI | Issue Writer Certificate

Keywords

NEURAL STEM CELLS (NSC)

NANOPARTICLES

TRANSFECTION

Abstract

Background: The magnetic nanoparticle-based TRANSFECTION method is a relatively new technique for delivery of functional genes to target tissues. We aimed to evaluate the TRANSFECTION efficiency of rat neural stem cell (NSC) using poly-L-lysine hydrobromide (PLL) -coated super paramagnetic iron oxide NANOPARTICLES (SPION).Methods: The SPION was prepared and coated with PLL as TRANSFECTION agent and the TRANSFECTION efficiency was evaluated in rat NSC using enhanced green fluorescent protein-N1 plasmid containing GFP as a reporter gene. NSC was incubated for 24 h in cell culture media containing 25 mg/ml SPION and in different concentrations of PLL (0.25, 0.50, 0.75, 1 and 2 mg/ml). Cell viability was determined before and after TRANSFECTION for every concentration using Trypan blue assay. Characterization of prepared uncoated (SPION) and coated (SPION-PLL) complexes were evaluated by a transmission electron microscope and the zeta potential.Results: PLL at 0.75 mg/ml showed optimal results with 25 mg/ml SPION concentration compared with other PLL concentrations (0.25, 0.50, 1 and 2 mg/ml). The 18% efficiency of the transfected cells showed green fluorescence.Conclusion: TRANSFECTION with SPION is an efficient, non-viral gene transfere method.

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APA: Copy

ALBUKHATY, SALIM, NADERI MANESH, HOSSEIN, & TIRAIHI, TAKI. (2013). IN VITRO LABELING OF NEURAL STEM CELLS WITH POLY-L-LYSINE COATED SUPER PARAMAGNETIC NANOPARTICLES FOR GREEN FLUORESCENT PROTEIN TRANSFECTION. IRANIAN BIOMEDICAL JOURNAL, 17(2), 71-76. SID. https://sid.ir/paper/277403/en

Vancouver: Copy

ALBUKHATY SALIM, NADERI MANESH HOSSEIN, TIRAIHI TAKI. IN VITRO LABELING OF NEURAL STEM CELLS WITH POLY-L-LYSINE COATED SUPER PARAMAGNETIC NANOPARTICLES FOR GREEN FLUORESCENT PROTEIN TRANSFECTION. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2013;17(2):71-76. Available from: https://sid.ir/paper/277403/en

IEEE: Copy

SALIM ALBUKHATY, HOSSEIN NADERI MANESH, and TAKI TIRAIHI, “IN VITRO LABELING OF NEURAL STEM CELLS WITH POLY-L-LYSINE COATED SUPER PARAMAGNETIC NANOPARTICLES FOR GREEN FLUORESCENT PROTEIN TRANSFECTION,” IRANIAN BIOMEDICAL JOURNAL, vol. 17, no. 2, pp. 71–76, 2013, [Online]. Available: https://sid.ir/paper/277403/en

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