COMPARATIVE STUDY OF IMMUNOLOGICAL AND STRUCTURAL PROPERTIES OF TWO RECOMBINANT VACCINE CANDIDATES AGAINST BOTULINUM NEUROTOXIN TYPE E

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ROSTAMIAN MOSAYEB; MOOSAVI SEYED JAFAR; EBRAHIMI FIROUZ; GHADAMI SEYYED ABOLGHASEM; SHEIBANI NADER; MINAEI MOHAMMAD EBRAHIM; AREFPOUR TORABI MOHAMMAD ALI

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Journal: Iranian Biomedical Journal Year:2012 | Volume:16 | Issue:4 Page(s): 185-192

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Title

COMPARATIVE STUDY OF IMMUNOLOGICAL AND STRUCTURAL PROPERTIES OF TWO RECOMBINANT VACCINE CANDIDATES AGAINST BOTULINUM NEUROTOXIN TYPE E

Author(s)

Keywords

BOTULINUM NEUROTOXIN TYPE E (BONT/E)

CROSS ELISA

CIRCULAR DICHROISM

COMPUTATIONAL MODELING

RECOMBINANT VACCINE-CANDIDATES

Abstract

Background: Recently, botulinum neurotoxin (BoNT)-derived recombinant proteins have been suggested as potential botulism vaccines. Here, with concentrating on BoNT type E (BoNT/E), we studied two of these binding domain-based recombinant proteins: a multivalent chimer protein, which is composed of BoNT serotypes A, B and E binding subdomains, and a monovalent recombinant protein, which contains 93 amino acid residues from recombinant C-terminal heavy chain of BoNT/E (rBoNT/E-HCC). Both proteins have an identical region (48 aa) that contains one of the most important BoNT/E epitopes (YLTHMRD sequence).Methods: The recombinant protein efficiency in antibody production, their structural differences, and their BoNT/E-epitope location were compared by using ELISA, CIRCULAR DICHROISM, COMPUTATIONAL MODELING, and hydrophobicity predictions.Results: Immunological studies indicated that the antibody yield against rBoNT/E-HCC was higher than chimer protein. CROSS ELISA confirmed that the antibodies against the chimer protein recognized rBoNT/E-HCC more efficiently. However, both antibody groups (anti-chimer and anti-rBoNT/E-HCC antibodies) were able to recognize other proteins. Structural studies with CIRCULAR DICHROISM showed that chimer proteins have slightly more secondary structures than rBoNT/E-HCC.Conclusion: The immunological results suggested that the above-mentioned identical region in rBoNT/E-HCC is more exposed. CIRCULAR DICHROISM, computational protein modeling and hydrophobicity predictions indicated a more exposed location for the identical region in rBoNT/E-HCC than the chimer protein, which is strongly in agreement with immunological results.

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APA: Copy

ROSTAMIAN, MOSAYEB, MOOSAVI, SEYED JAFAR, EBRAHIMI, FIROUZ, GHADAMI, SEYYED ABOLGHASEM, SHEIBANI, NADER, MINAEI, MOHAMMAD EBRAHIM, & AREFPOUR TORABI, MOHAMMAD ALI. (2012). COMPARATIVE STUDY OF IMMUNOLOGICAL AND STRUCTURAL PROPERTIES OF TWO RECOMBINANT VACCINE CANDIDATES AGAINST BOTULINUM NEUROTOXIN TYPE E. IRANIAN BIOMEDICAL JOURNAL, 16(4), 185-192. SID. https://sid.ir/paper/277424/en

Vancouver: Copy

ROSTAMIAN MOSAYEB, MOOSAVI SEYED JAFAR, EBRAHIMI FIROUZ, GHADAMI SEYYED ABOLGHASEM, SHEIBANI NADER, MINAEI MOHAMMAD EBRAHIM, AREFPOUR TORABI MOHAMMAD ALI. COMPARATIVE STUDY OF IMMUNOLOGICAL AND STRUCTURAL PROPERTIES OF TWO RECOMBINANT VACCINE CANDIDATES AGAINST BOTULINUM NEUROTOXIN TYPE E. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2012;16(4):185-192. Available from: https://sid.ir/paper/277424/en

IEEE: Copy

MOSAYEB ROSTAMIAN, SEYED JAFAR MOOSAVI, FIROUZ EBRAHIMI, SEYYED ABOLGHASEM GHADAMI, NADER SHEIBANI, MOHAMMAD EBRAHIM MINAEI, and MOHAMMAD ALI AREFPOUR TORABI, “COMPARATIVE STUDY OF IMMUNOLOGICAL AND STRUCTURAL PROPERTIES OF TWO RECOMBINANT VACCINE CANDIDATES AGAINST BOTULINUM NEUROTOXIN TYPE E,” IRANIAN BIOMEDICAL JOURNAL, vol. 16, no. 4, pp. 185–192, 2012, [Online]. Available: https://sid.ir/paper/277424/en

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