مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

EFFECTS OF PEPTONE SUPPLEMENTATION IN DIFFERENT CULTURE MEDIA ON GROWTH, METABOLIC PATHWAY AND PRODUCTIVITY OF CHO DG44 CELLS; A NEW INSIGHT INTO AMINO ACID PROFILES

Pages

  194-205

Abstract

 Background: The optimization of bioprocess conditions towards improved growth profile and productivity yield is considered of great importance in biopharmaceutical manufacturing. PEPTONES as efficient sources of nutrients have been studied for their effect on media development; however, their role on metabolic pathway is not well understood. Methods: In the present study, the effect of different concentration of PEPTONES on a recombinant Chinese hamster ovary (CHO) cell line grown in three serum-free suspension cultures was determined. Six PEPTONES of different origins and available amino acid profiles were investigated regarding their impact on cell growth, productivity, and metabolic pathways changes. Results: In optimized feeding strategies, increases of 136% and 159% in volumetric productivity (for a low-nutrient CULTURE MEDIA) and 55% (for a high-nutrient CULTURE MEDIA) were achieved. Furthermore, particular sources of PEPTONES with specific amino acid profile developed preferential results for each different culture medium. Two PEPTONES, SoyA2SC and SoyE-110, were the only hydro lysates that showed production improvement in all three media. Casein Peptone plus Tryptone N1 and SoyA3SC showed different improved results based on their implemented concentration for each individual basal medium. Conclusion: The amino acid profile of PEPTONES may provide clues to identify the most effective feeding strategies for recombinant CHO CELLS.

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    APA: Copy

    DAVAMI, FATEMEH, EGHBALPOUR, FARNAZ, NEMATOLLAHI, LEILA, BARKHORDARI, FARZANEH, & MAHBOUDI, FEREIDOUN. (2015). EFFECTS OF PEPTONE SUPPLEMENTATION IN DIFFERENT CULTURE MEDIA ON GROWTH, METABOLIC PATHWAY AND PRODUCTIVITY OF CHO DG44 CELLS; A NEW INSIGHT INTO AMINO ACID PROFILES. IRANIAN BIOMEDICAL JOURNAL, 19(4), 194-205. SID. https://sid.ir/paper/277443/en

    Vancouver: Copy

    DAVAMI FATEMEH, EGHBALPOUR FARNAZ, NEMATOLLAHI LEILA, BARKHORDARI FARZANEH, MAHBOUDI FEREIDOUN. EFFECTS OF PEPTONE SUPPLEMENTATION IN DIFFERENT CULTURE MEDIA ON GROWTH, METABOLIC PATHWAY AND PRODUCTIVITY OF CHO DG44 CELLS; A NEW INSIGHT INTO AMINO ACID PROFILES. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2015;19(4):194-205. Available from: https://sid.ir/paper/277443/en

    IEEE: Copy

    FATEMEH DAVAMI, FARNAZ EGHBALPOUR, LEILA NEMATOLLAHI, FARZANEH BARKHORDARI, and FEREIDOUN MAHBOUDI, “EFFECTS OF PEPTONE SUPPLEMENTATION IN DIFFERENT CULTURE MEDIA ON GROWTH, METABOLIC PATHWAY AND PRODUCTIVITY OF CHO DG44 CELLS; A NEW INSIGHT INTO AMINO ACID PROFILES,” IRANIAN BIOMEDICAL JOURNAL, vol. 19, no. 4, pp. 194–205, 2015, [Online]. Available: https://sid.ir/paper/277443/en

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