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Information Journal Paper

Title

INHIBITION ASSAY STUDY OF PURIFIED GLUTATHIONE S-TRANSFERASE FROM FASCIOLA HEPATICA AND SHEEP LIVER TISSUE BY HEXACHLOROPHENE

Pages

  168-171

Abstract

 Glutathione S-transferases (GSTs) are widespread in Fasciola. hepatica parasite and sheep liver tissue. Study of GSTs INHIBITION ASSAYs in F. hepatica and sheep liver tissue are a priority of chemotherapeutic targets in parasitic liver diseases including human fascioliasis in Iran. In this research, the whole extract of F. hepatica and sheep liver tissues were purified and eluted for sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) pattern and GSTs INHIBITION ASSAY. GSTs inhibition was detected by hexachlorophene as an inhibitor and 1-chloro-2,4-dinitrobenzene (CDNB) as secondary substrate. The purified GSTs from F. hepatica and liver tissue contained comparable components and showed a molecular weight of 26kDa. The inhibitor concentration of hexachlorophene, for the remaining 50% activity (IC50%) of GST enzymes from F. hepatica and liver were graphically calculated, and the results were 0.25 µM and 1 µM, respectively. GSTs of F. hepatica may be more sensitive than sheep liver tissue to hexachlorophene

Cites

References

Cite

APA: Copy

FARAHNAK, A., & BROPHY, P.M.. (2004). INHIBITION ASSAY STUDY OF PURIFIED GLUTATHIONE S-TRANSFERASE FROM FASCIOLA HEPATICA AND SHEEP LIVER TISSUE BY HEXACHLOROPHENE. ACTA MEDICA IRANICA, 42(3), 168-171. SID. https://sid.ir/paper/277602/en

Vancouver: Copy

FARAHNAK A., BROPHY P.M.. INHIBITION ASSAY STUDY OF PURIFIED GLUTATHIONE S-TRANSFERASE FROM FASCIOLA HEPATICA AND SHEEP LIVER TISSUE BY HEXACHLOROPHENE. ACTA MEDICA IRANICA[Internet]. 2004;42(3):168-171. Available from: https://sid.ir/paper/277602/en

IEEE: Copy

A. FARAHNAK, and P.M. BROPHY, “INHIBITION ASSAY STUDY OF PURIFIED GLUTATHIONE S-TRANSFERASE FROM FASCIOLA HEPATICA AND SHEEP LIVER TISSUE BY HEXACHLOROPHENE,” ACTA MEDICA IRANICA, vol. 42, no. 3, pp. 168–171, 2004, [Online]. Available: https://sid.ir/paper/277602/en

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