INHIBITORY EFFECT OF GABA ON GLUCOSE- STIMULATED INSULIN SECRETION FROM ISOLATED ISLETS OF LANGERHANS IN RAT

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FARAJI FARZANEH; GHASEMI ASGHAR; KHOSRAVI MARYAM; MOTAMEDI F.; ZAHEDIASL S.

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Journal: Iranian Journal of Endocrinology & Metabolism Year:2008 | Volume:10 | Issue:1 (SN 37) Page(s): 59-66

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Title

INHIBITORY EFFECT OF GABA ON GLUCOSE- STIMULATED INSULIN SECRETION FROM ISOLATED ISLETS OF LANGERHANS IN RAT

Author(s)

Keywords

GABAQ3

BACLOFENQ3

SACLOFENQ3

INSULINQ3

GLUCOSEQ3

LANGERHANS ISLETSQ3

RATSQ2

Abstract

Introduction: Gamma-amino butyric acid (GABA), an amino acid, present in some inhibitory neurons of the central nervous system, is also found in relatively high levels in the islets of Langerhans. Results of different studies concerning the effect of GABA on INSULIN secretion are controversial. The aim of this study was to determine the role of GABA and GABAB receptors on GLUCOSE-stimulated INSULIN secretion in isolated islets of RATS. Materials and Methods: The collagenase digestion technique was used to isolate the islets from pancreata of 45 male Wistar RATS (200-250g). INSULIN secretion was assessed in eight islets in each cup exposed to different concentrations of GLUCOSE (8.3 and 16.7 mM) in the presence or absence of GABA (25, 50, 100 mM), BACLOFEN (10, 20, 50 mM) (GABAB agonist) and SACLOFEN (50,100 mM) (GABAB antagonist). INSULIN concentration was measured by the ELISA method. INSULIN release was reported as mean±SEM mU/islet/50min and p values of <0.05 were considered significant. Results: GABA inhibited GLUCOSE (8.3 and 16.7 mM)-induced INSULIN secretion in isolated islets (P<0.05). Different concentrations of BACLOFEN had no significant effect on GLUCOSE-induced INSULIN secretion; however GLUCOSE (16.7mM) stimulated INSULIN secretion in the presence of 100 mM SACLOFEN (91±8.8 mU/islet/60min) was significantly (p<0.05) higher compared to INSULIN secretion stimulated by 16.7mM GLUCOSE alone (67.7±2.58 mU/islet/60min). Conclusion: These findings indicate that GABA has an inhibitory effect on GLUCOSE-induced INSULIN secretion; and therefore it may play a regulatory role in INSULIN secretion. This effect needs to be taken in to account in the pathophysiology of diabetes.

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APA: Copy

FARAJI, FARZANEH, GHASEMI, ASGHAR, KHOSRAVI, MARYAM, MOTAMEDI, F., & ZAHEDIASL, S.. (2008). INHIBITORY EFFECT OF GABA ON GLUCOSE- STIMULATED INSULIN SECRETION FROM ISOLATED ISLETS OF LANGERHANS IN RAT. IRANIAN JOURNAL OF ENDOCRINOLOGY AND METABOLISM (IJEM), 10(1 (SN 37)), 59-66. SID. https://sid.ir/paper/27918/en

Vancouver: Copy

FARAJI FARZANEH, GHASEMI ASGHAR, KHOSRAVI MARYAM, MOTAMEDI F., ZAHEDIASL S.. INHIBITORY EFFECT OF GABA ON GLUCOSE- STIMULATED INSULIN SECRETION FROM ISOLATED ISLETS OF LANGERHANS IN RAT. IRANIAN JOURNAL OF ENDOCRINOLOGY AND METABOLISM (IJEM)[Internet]. 2008;10(1 (SN 37)):59-66. Available from: https://sid.ir/paper/27918/en

IEEE: Copy

FARZANEH FARAJI, ASGHAR GHASEMI, MARYAM KHOSRAVI, F. MOTAMEDI, and S. ZAHEDIASL, “INHIBITORY EFFECT OF GABA ON GLUCOSE- STIMULATED INSULIN SECRETION FROM ISOLATED ISLETS OF LANGERHANS IN RAT,” IRANIAN JOURNAL OF ENDOCRINOLOGY AND METABOLISM (IJEM), vol. 10, no. 1 (SN 37), pp. 59–66, 2008, [Online]. Available: https://sid.ir/paper/27918/en

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