DETECTION OF PLASMODIUM PARASITES IN HEALTHY BLOOD DONORS USING POLYMERASE CHAIN REACTION

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KAZEMI B.; NAJARI M.; SANEI MOGHADAM E.; BANDEHPOUR M.; SEYED N.; SHARIFI KH.

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Journal Paper

Paper Information

Journal: ARCHIVES OF IRANIAN MEDICINE Year:2005 | Volume:8 | Issue:2 Page(s): 135-138

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Title

DETECTION OF PLASMODIUM PARASITES IN HEALTHY BLOOD DONORS USING POLYMERASE CHAIN REACTION

Author(s)

Keywords

BLOOD DONORS • IRAN • MALARIA • PCR-RFLP • TRANSFUSION MALARIA

Abstract

Malaria is an important tropical disease in terms of morbidity and mortality. It is difficult to
identify hidden contaminated cases through the use of ordinary techniques, such as microscopic
methods, in endemic regions. In the present study, two techniques were used to assess the rate of
contingent malaria contamination in healthy inhabitants: microscopic study (thin and thick blood
smears) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
One hundred blood samples were collected from healthy individuals who lived in Chabahar,
southeastern Iran, in June 2002, during peak mosquito season.
Three positive samples (3%) were detected by PCR method, all of which had been reported
negative by microscopic examination. It seems that the most appropriate method for populationbased
screening of blood donors for malaria parasites could be through the detection of parasite
nucleic acids using PCR. Such a strategy would also reduce transfusion-transmitted malaria in
endemic areas.

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APA: Copy

KAZEMI, B., NAJARI, M., SANEI MOGHADAM, E., BANDEHPOUR, M., SEYED, N., & SHARIFI, KH.. (2005). DETECTION OF PLASMODIUM PARASITES IN HEALTHY BLOOD DONORS USING POLYMERASE CHAIN REACTION. ARCHIVES OF IRANIAN MEDICINE, 8(2), 135-138. SID. https://sid.ir/paper/280032/en

Vancouver: Copy

KAZEMI B., NAJARI M., SANEI MOGHADAM E., BANDEHPOUR M., SEYED N., SHARIFI KH.. DETECTION OF PLASMODIUM PARASITES IN HEALTHY BLOOD DONORS USING POLYMERASE CHAIN REACTION. ARCHIVES OF IRANIAN MEDICINE[Internet]. 2005;8(2):135-138. Available from: https://sid.ir/paper/280032/en

IEEE: Copy

B. KAZEMI, M. NAJARI, E. SANEI MOGHADAM, M. BANDEHPOUR, N. SEYED, and KH. SHARIFI, “DETECTION OF PLASMODIUM PARASITES IN HEALTHY BLOOD DONORS USING POLYMERASE CHAIN REACTION,” ARCHIVES OF IRANIAN MEDICINE, vol. 8, no. 2, pp. 135–138, 2005, [Online]. Available: https://sid.ir/paper/280032/en

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