CLONING AND EXPRESSION OF HEPATITIS C VIRUS CORE PROTEIN IN PGEMEX-1 EXPRESSION VECTOR

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

KAZEMI B.; BANDEHPOUR M.; SEYED N.; ROUZBEHI M.; MOSAFA N.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: ARCHIVES OF IRANIAN MEDICINE Year:2008 | Volume:11 | Issue:2 Page(s): 173-178

Download Full-Text

View:

350

Download:

362

Cites:

Information Journal Paper

Title

CLONING AND EXPRESSION OF HEPATITIS C VIRUS CORE PROTEIN IN PGEMEX-1 EXPRESSION VECTOR

Author(s)

Keywords

GENE EXPRESSION

HEPATITIS C VIRUS

Abstract

Background: HEPATITIS C VIRUS is one of the main causes of chronic hepatitis in developing countries. There are 170 million affected people around the world as reported by the World Health Organization. The treatment of hepatitis C is not successful in most cases; it is extremely costly, and requires prolonged therapy, therefore it is desirable to develop a vaccine to prevent the spread of HEPATITIS C VIRUS. Methods: HEPATITIS C VIRUS RNA was extracted from a HEPATITIS C VIRUS-infected serum sample. cDNA was synthesized and the HEPATITIS C VIRUS core gene was amplified by polymerase chain reaction. The polymerase chain reaction product was cloned in pGEMEX-1 expression vector and expressed in Escherichia coli BL21 strain with DE3 (a l prophage carrying the T7 RNA polymerase gene) host by induction of promoter using one millimolar isopropyl b-Dthiogalactopyranoside in laboratory scale. Induced lysate cells were electrophoresed on SDS polyacrylamide gel.Results: A protein band was detected in induced cells in comparison with non-induced cells. Expressed protein was confirmed by gel diffusion and dot blot analysis using induced lysate cells as antigen and HEPATITIS C VIRUS-infected serum as antibody.Conclusion: In present study, we have provided a recombinant plasmid based on HEPATITIS C VIRUS core gene.

Cites

No record.

References

No record.

Cite

APA: Copy

KAZEMI, B., BANDEHPOUR, M., SEYED, N., ROUZBEHI, M., & MOSAFA, N.. (2008). CLONING AND EXPRESSION OF HEPATITIS C VIRUS CORE PROTEIN IN PGEMEX-1 EXPRESSION VECTOR. ARCHIVES OF IRANIAN MEDICINE, 11(2), 173-178. SID. https://sid.ir/paper/280293/en

Vancouver: Copy

KAZEMI B., BANDEHPOUR M., SEYED N., ROUZBEHI M., MOSAFA N.. CLONING AND EXPRESSION OF HEPATITIS C VIRUS CORE PROTEIN IN PGEMEX-1 EXPRESSION VECTOR. ARCHIVES OF IRANIAN MEDICINE[Internet]. 2008;11(2):173-178. Available from: https://sid.ir/paper/280293/en

IEEE: Copy

B. KAZEMI, M. BANDEHPOUR, N. SEYED, M. ROUZBEHI, and N. MOSAFA, “CLONING AND EXPRESSION OF HEPATITIS C VIRUS CORE PROTEIN IN PGEMEX-1 EXPRESSION VECTOR,” ARCHIVES OF IRANIAN MEDICINE, vol. 11, no. 2, pp. 173–178, 2008, [Online]. Available: https://sid.ir/paper/280293/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops