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Information Journal Paper

Title

GENOTYPE ANALYSIS OF HEPATITIS DELTA VIRUS FROM HEPATITIS B SURFACE ANTIGEN-POSITIVE PATIENTS USING PCR-RFLP IN TEHRAN, IRAN

Pages

  238-243

Abstract

 Background: HEPATITIS DELTA VIRUS is a unique human pathogen responsible for some 20 million infections globally. This virus is dependent on hepatitis B virus for transmission and propagation. Currently, at least three genotypes of HEPATITIS DELTA VIRUS with different geographic distribution and clinical manifestations are described.Methods: In this study, HEPATITIS DELTA VIRUS RNA of 35 patients’ sera were analyzed by RTsemi- nested polymerase chain reaction. Based on genomic differences of hepatitis delta antigen coding region of HEPATITIS DELTA VIRUS RNA among HEPATITIS DELTA VIRUS RNA-positive sera, the polymerase chain reaction products were digested with restriction enzymes and studied by restriction fragment length polymorphism.Results: Out of 35 samples, 13 (38.46%) were positive for HEPATITIS DELTA VIRUS RNA by RTsemi- nested polymerase chain reaction. All polymorphisms were shown to be genotype I. Out of 13 HEPATITIS DELTA VIRUS RNA-positive (13/35), eight were HBeAg negative.Conclusion: Our data indicated that HEPATITIS DELTA VIRUS isolates in TEHRAN are exclusively genotype I.

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    APA: Copy

    MIRSHAFIEI, H., MAHMOUDIAN SHOUSHTARI, M., SHARIFI, ZOHREH, & HOSSEINI, SEYED MASOUD. (2009). GENOTYPE ANALYSIS OF HEPATITIS DELTA VIRUS FROM HEPATITIS B SURFACE ANTIGEN-POSITIVE PATIENTS USING PCR-RFLP IN TEHRAN, IRAN. ARCHIVES OF IRANIAN MEDICINE, 12(3), 238-243. SID. https://sid.ir/paper/280457/en

    Vancouver: Copy

    MIRSHAFIEI H., MAHMOUDIAN SHOUSHTARI M., SHARIFI ZOHREH, HOSSEINI SEYED MASOUD. GENOTYPE ANALYSIS OF HEPATITIS DELTA VIRUS FROM HEPATITIS B SURFACE ANTIGEN-POSITIVE PATIENTS USING PCR-RFLP IN TEHRAN, IRAN. ARCHIVES OF IRANIAN MEDICINE[Internet]. 2009;12(3):238-243. Available from: https://sid.ir/paper/280457/en

    IEEE: Copy

    H. MIRSHAFIEI, M. MAHMOUDIAN SHOUSHTARI, ZOHREH SHARIFI, and SEYED MASOUD HOSSEINI, “GENOTYPE ANALYSIS OF HEPATITIS DELTA VIRUS FROM HEPATITIS B SURFACE ANTIGEN-POSITIVE PATIENTS USING PCR-RFLP IN TEHRAN, IRAN,” ARCHIVES OF IRANIAN MEDICINE, vol. 12, no. 3, pp. 238–243, 2009, [Online]. Available: https://sid.ir/paper/280457/en

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