EVALUATION OF FAST-ELISA VERSUS STANDARD-ELISA TO DIAGNOSE HUMAN FASCIOLOSIS

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RAHIMI MOHAMMAD TAGHI; ASHRAFI KAYHAN; KOOSHA SOHEYLA; ABDI JAHANGIR; ROKNI MOHAMMAD BAGHER

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Paper Information

Journal: ARCHIVES OF IRANIAN MEDICINE Year:2011 | Volume:14 | Issue:1 Page(s): 18-21

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Title

EVALUATION OF FAST-ELISA VERSUS STANDARD-ELISA TO DIAGNOSE HUMAN FASCIOLOSIS

Author(s)

Keywords

DIAGNOSIS

ENZYME-LINKED IMMUNOSORBENT ASSAY

FASCIOLA

VALIDITY

Abstract

Background: The present study was conducted to evaluate and compare a fast-ELISA (F-ELISA) method versus standard- ELISA (S-ELISA) to diagnose human fasciolosis.Methods: Serum samples were obtained from 35 individuals infected with fasciolosis, 27 infected with other parasitoses and 22 from healthy people. The samples were examined with S-ELISA (30-minute incubation periods) and F-ELISA (10 minute incubation periods) for total antibody response against fasciolosis.Results: The optimum conditions for S-ELISA and F-ELISA were respectively as follows: antigen 10 and 5 mg/mL, sera 1:500 dilution for both, peroxidase-conjugated goat anti-human IgG diluted 1:7000 and 1:10000. Data were analyzed using SPSS version 11.0. Cut-off vale for S-ELISA and F-ELISA was determined as 0.56 and 0.42, respectively. The sensitivity, specificity; positive, and negative predictive values were detected as 97.2%, 100%, 94.6%, and 95.6% for both tests.Conclusion: Cut-off values, sensitivity, specificity, and other important parameters of the two evaluated tests determined that the F-ELISA method could be used with no detectable difference.

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APA: Copy

RAHIMI, MOHAMMAD TAGHI, ASHRAFI, KAYHAN, KOOSHA, SOHEYLA, ABDI, JAHANGIR, & ROKNI, MOHAMMAD BAGHER. (2011). EVALUATION OF FAST-ELISA VERSUS STANDARD-ELISA TO DIAGNOSE HUMAN FASCIOLOSIS. ARCHIVES OF IRANIAN MEDICINE, 14(1), 18-21. SID. https://sid.ir/paper/280720/en

Vancouver: Copy

RAHIMI MOHAMMAD TAGHI, ASHRAFI KAYHAN, KOOSHA SOHEYLA, ABDI JAHANGIR, ROKNI MOHAMMAD BAGHER. EVALUATION OF FAST-ELISA VERSUS STANDARD-ELISA TO DIAGNOSE HUMAN FASCIOLOSIS. ARCHIVES OF IRANIAN MEDICINE[Internet]. 2011;14(1):18-21. Available from: https://sid.ir/paper/280720/en

IEEE: Copy

MOHAMMAD TAGHI RAHIMI, KAYHAN ASHRAFI, SOHEYLA KOOSHA, JAHANGIR ABDI, and MOHAMMAD BAGHER ROKNI, “EVALUATION OF FAST-ELISA VERSUS STANDARD-ELISA TO DIAGNOSE HUMAN FASCIOLOSIS,” ARCHIVES OF IRANIAN MEDICINE, vol. 14, no. 1, pp. 18–21, 2011, [Online]. Available: https://sid.ir/paper/280720/en

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