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Title

MOLECULAR PATHOLOGY AND HISTOPATHOLOGICAL FINDINGS IN LOCALIZED LEISHMANIA LYMPHADENITIS

Pages

  122-126

Abstract

 BACKGROUND: A rare variant of Leishmaniasis is LOCALIZED LEISHMANIA LYMPHADENITIS which has been occasionally reported from south-eastern parts of Iran. So far, no molecular assay has been performed for diagnosing this variety of Leishmaniasis.MATHERIALS AND METHODS: Nineteen lymph node paraffin blocks were collected from 1994 to 2007. Parasite load count and histopathological patterns reported on Hematoxylin-Eosin and Giemsa stained slides.DNA extraction was carried out just on the remaining available 7 lymph node paraffin blocks according to QIAamp DNA FFPE kit instructions. A pair of primers and a probe were designed for rRNA ITS region with Allele ID 6.0 software, followed by REAL TIME PCR amplification.RESULT: The most common histopathological pattern was necrotizing granuloma with few Leishman bodies. Parasite load was the highest in submental lymph node (3±1.41 per oil field) which was significantly higher compared to cervical and inguinal nodes (P<0.05). Absolute load of parasite DNA was detectable in all 7 cases. The positive cases revealed a 201 bpamplicon after electrophoresis of end product which was confirmative for LEISHMANIA TROPICA.CONCLUSION: REAL TIME PCR revealed LEISHMANIA TROPICA as the etiologic agent of LOCALIZED LEISHMANIA LYMPHADENITIS. Although this molecular method is a sensitive diagnostic tool, histopathological findings are still important.

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    APA: Copy

    DABIRI, SHAHRIAR, SAFAVI, MOEINADIN, SHAMSI MEYMANDI, SIMIN, YOUSEFI, KERAMAT, SHAMSI MEYMANDI, MANZUMEH, FOTOUHI ARDAKANI, REZA, & FEKRI SOOFIABADI, MARYAM. (2014). MOLECULAR PATHOLOGY AND HISTOPATHOLOGICAL FINDINGS IN LOCALIZED LEISHMANIA LYMPHADENITIS. ARCHIVES OF IRANIAN MEDICINE, 17(2), 122-126. SID. https://sid.ir/paper/280877/en

    Vancouver: Copy

    DABIRI SHAHRIAR, SAFAVI MOEINADIN, SHAMSI MEYMANDI SIMIN, YOUSEFI KERAMAT, SHAMSI MEYMANDI MANZUMEH, FOTOUHI ARDAKANI REZA, FEKRI SOOFIABADI MARYAM. MOLECULAR PATHOLOGY AND HISTOPATHOLOGICAL FINDINGS IN LOCALIZED LEISHMANIA LYMPHADENITIS. ARCHIVES OF IRANIAN MEDICINE[Internet]. 2014;17(2):122-126. Available from: https://sid.ir/paper/280877/en

    IEEE: Copy

    SHAHRIAR DABIRI, MOEINADIN SAFAVI, SIMIN SHAMSI MEYMANDI, KERAMAT YOUSEFI, MANZUMEH SHAMSI MEYMANDI, REZA FOTOUHI ARDAKANI, and MARYAM FEKRI SOOFIABADI, “MOLECULAR PATHOLOGY AND HISTOPATHOLOGICAL FINDINGS IN LOCALIZED LEISHMANIA LYMPHADENITIS,” ARCHIVES OF IRANIAN MEDICINE, vol. 17, no. 2, pp. 122–126, 2014, [Online]. Available: https://sid.ir/paper/280877/en

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