A COMPARATIVE APPROACH BETWEEN HETEROLOGOUS PRIME-BOOST VACCINATION STRATEGY AND DNA VACCINATIONS FOR RABIES

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BORHANI KIANDOKHT; AJORLOO MEHDI; BAMDAD TARAVAT; MOZHGANI SAYED HAMID REZA; GHADERI MOSTAFA; GHOLAMI ALIREZA

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Journal: ARCHIVES OF IRANIAN MEDICINE Year:2015 | Volume:18 | Issue:4 Page(s): 223-227

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Title

A COMPARATIVE APPROACH BETWEEN HETEROLOGOUS PRIME-BOOST VACCINATION STRATEGY AND DNA VACCINATIONS FOR RABIES

Author(s)

Keywords

DNA VACCINE

PASTEUR VIRUS

PRIME

BOOST

RABIES

Abstract

BACKGROUND AND AIM: RABIES is a widespread neurological zoonotic disease causing significant mortality rates, especially in developing countries. Although a vaccine for RABIES is available, its production and scheduling are costly in such countries. Advances in recombinant DNA technology have made it a good candidate for an affordable vaccine. Among the proteins of RABIES virus, the Glycoprotein (RVG) has been the major target for new vaccine development which plays the principalrole in providing complete protection against RV challenge. The aim of this study is to produce recombinant RVG which could be a DNA VACCINE candidate and to evaluate the efficiency of this construct in a PRIME-boost vaccination regimen, compared to commercial vaccine.METHODS: Cloning to pcDNA3.1 (+) and expression of RABIES virus glycoprotein gene in BSR cell line were performed followed by SDS-PAGE and Western blot analysis of the expressed glycoprotein. The resulting genetic construct was used as a DNA VACCINE by injecting 80 mg of the plasmid to MNRI mice twice. PRIME-Boost vaccination strategy was performed using 80 mg plasmid construct as PRIME dose and the second dose of an inactivated RABIES virus vaccine. Production of RABIES virus neutralizing antibody (RVNA) titers of the serum samples were determined by RFFIT.RESULTS: In comparisons between heterologous PRIME-boost vaccination strategy and DNA vaccinations, the potency of group D that received PRIME-Boost vaccine with the second dose of pcDNA3.1 (+)-Gp was enhanced significantly compared to the group C which had received pcDNA3.1 (+)-Gp as first injection.CONCLUSION: In this study, RVGP expressing construct was used in a comparative approach between PRIME-Boost vaccination strategy and DNA vaccination and compared with the standard method of RABIES vaccination. It was concluded that this strategy could lead to induction of acceptable humoral immunity.

Cites

ASSESSMENT THE EFFICIENCY OF THE CONSTRUCTED MINIGENOME OF RABIES VIRUS USING PV STRAIN AS HELPER VIRUS

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APA: Copy

BORHANI, KIANDOKHT, AJORLOO, MEHDI, BAMDAD, TARAVAT, MOZHGANI, SAYED HAMID REZA, GHADERI, MOSTAFA, & GHOLAMI, ALIREZA. (2015). A COMPARATIVE APPROACH BETWEEN HETEROLOGOUS PRIME-BOOST VACCINATION STRATEGY AND DNA VACCINATIONS FOR RABIES. ARCHIVES OF IRANIAN MEDICINE, 18(4), 223-227. SID. https://sid.ir/paper/281224/en

Vancouver: Copy

BORHANI KIANDOKHT, AJORLOO MEHDI, BAMDAD TARAVAT, MOZHGANI SAYED HAMID REZA, GHADERI MOSTAFA, GHOLAMI ALIREZA. A COMPARATIVE APPROACH BETWEEN HETEROLOGOUS PRIME-BOOST VACCINATION STRATEGY AND DNA VACCINATIONS FOR RABIES. ARCHIVES OF IRANIAN MEDICINE[Internet]. 2015;18(4):223-227. Available from: https://sid.ir/paper/281224/en

IEEE: Copy

KIANDOKHT BORHANI, MEHDI AJORLOO, TARAVAT BAMDAD, SAYED HAMID REZA MOZHGANI, MOSTAFA GHADERI, and ALIREZA GHOLAMI, “A COMPARATIVE APPROACH BETWEEN HETEROLOGOUS PRIME-BOOST VACCINATION STRATEGY AND DNA VACCINATIONS FOR RABIES,” ARCHIVES OF IRANIAN MEDICINE, vol. 18, no. 4, pp. 223–227, 2015, [Online]. Available: https://sid.ir/paper/281224/en

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