A SIMPLE AND COST-EFFECTIVE METHOD FOR ISOLATION AND EXPANSION OF HUMAN FETAL PANCREAS DERIVED MESENCHYMAL STEM CELLS

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LARIJANI BAGHER; ARJMAND BABAK; AHMADBEIGI NASER; FALAHZADEH KHADIJEH; SOLEIMANI MASOUD; SAYAHPOUR FOROUGH AZAM; AGHAYAN HAMID REZA

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Journal: ARCHIVES OF IRANIAN MEDICINE Year:2015 | Volume:18 | Issue:11 Page(s): 770-775

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Title

A SIMPLE AND COST-EFFECTIVE METHOD FOR ISOLATION AND EXPANSION OF HUMAN FETAL PANCREAS DERIVED MESENCHYMAL STEM CELLS

Author(s)

Keywords

CELL TRANSPLANTATION

CLINICAL GRADE

DIABETES

FETAL PANCREAS

MESENCHYMAL STEM CELL

Abstract

BACKGROUND: Previous studies have suggested MESENCHYMAL STEM CELLs (MSCs) as a suitable source for cell replacement therapy in DIABETES. MSCs have successfully isolated from different adult and fetal tissues, including the pancreas. In vitro studies have shown that human fetal pancreatic stem cells could be extensively expanded and differentiated into islet-like structures. Here, we introduce a simple and cost-effective method for the generation of MSCs from the human FETAL PANCREAS (FPMSCs).METHODS: To isolate FPMSCs, pancreata from four aborted fetuses (second trimester) were processed with short collagenase digestion. The resulting tissue fragments were transferred to a basic media (DMEM+15%FBS) without adding any growth factor.RESULTS: After 10 to14 days, fibroblast-like cells were harvested and passaged six times for further evaluations. Flow cytometry analysis and three-lineage differentiation capacity have demonstrated that these cells have MSC-like properties. We also continuously passaged samples of FPMSCs and found no evidence for chromosomal instability and morphological changes until 10th subculture. Moreover, our cell culture protocol can be easily modified and translated into a GMP-compliant one.CONCLUSION: The results of current study demonstrated that our simple and inexpensive method could yield a pure population of FPMSCs that might be suitable for transplantation.

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APA: Copy

LARIJANI, BAGHER, ARJMAND, BABAK, AHMADBEIGI, NASER, FALAHZADEH, KHADIJEH, SOLEIMANI, MASOUD, SAYAHPOUR, FOROUGH AZAM, & AGHAYAN, HAMID REZA. (2015). A SIMPLE AND COST-EFFECTIVE METHOD FOR ISOLATION AND EXPANSION OF HUMAN FETAL PANCREAS DERIVED MESENCHYMAL STEM CELLS. ARCHIVES OF IRANIAN MEDICINE, 18(11), 770-775. SID. https://sid.ir/paper/281297/en

Vancouver: Copy

LARIJANI BAGHER, ARJMAND BABAK, AHMADBEIGI NASER, FALAHZADEH KHADIJEH, SOLEIMANI MASOUD, SAYAHPOUR FOROUGH AZAM, AGHAYAN HAMID REZA. A SIMPLE AND COST-EFFECTIVE METHOD FOR ISOLATION AND EXPANSION OF HUMAN FETAL PANCREAS DERIVED MESENCHYMAL STEM CELLS. ARCHIVES OF IRANIAN MEDICINE[Internet]. 2015;18(11):770-775. Available from: https://sid.ir/paper/281297/en

IEEE: Copy

BAGHER LARIJANI, BABAK ARJMAND, NASER AHMADBEIGI, KHADIJEH FALAHZADEH, MASOUD SOLEIMANI, FOROUGH AZAM SAYAHPOUR, and HAMID REZA AGHAYAN, “A SIMPLE AND COST-EFFECTIVE METHOD FOR ISOLATION AND EXPANSION OF HUMAN FETAL PANCREAS DERIVED MESENCHYMAL STEM CELLS,” ARCHIVES OF IRANIAN MEDICINE, vol. 18, no. 11, pp. 770–775, 2015, [Online]. Available: https://sid.ir/paper/281297/en

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