DOPAMINERGIC INDUCTION OF UMBILICAL CORD MESENCHYMAL STEM CELLS BY CONDITIONED MEDIUM OF CHOROID PLEXUS EPITHELIAL CELLS REDUCES APOMORPHINE-INDUCED ROTATION IN PARKINSONIAN RATS

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ALIAGHAEI A.; GARDANEH MOSSA; MAGHSOUDI NADER; SALEHINEJAD PARVIN; GHARIB EHSAN

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Journal Paper

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Journal: ARCHIVES OF IRANIAN MEDICINE Year:2016 | Volume:19 | Issue:8 Page(s): 561-570

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Title

DOPAMINERGIC INDUCTION OF UMBILICAL CORD MESENCHYMAL STEM CELLS BY CONDITIONED MEDIUM OF CHOROID PLEXUS EPITHELIAL CELLS REDUCES APOMORPHINE-INDUCED ROTATION IN PARKINSONIAN RATS

Author(s)

Keywords

CHOROID PLEXUS

DOPAMINERGIC

MESENCHYMAL STEM CELLS

PARKINSON

UMBILICAL CORD

Abstract

BACKGROUND/OBJECTIVE: Degeneration of DOPAMINERGIC neurons in PARKINSON’s disease (PD) implies cell replacement using potentially differentiable sources as a promising therapeutic solution. We tested the capacity of conditioned medium from CHOROID PLEXUS epithelial cells (CPECs-CM) to induce the DOPAMINERGIC potential of UMBILICAL CORD matrix MESENCHYMAL STEM CELLS (UCMSCs). METHODS: We isolated UCMSCs from human UMBILICAL CORD and CPECs from rat brain. Following expansion and characterization, CPECs-CM were collected, tested for expression of various growth factors, and applied to UCMSCs. Differentiation was examined and UCMSCs were injected into 6-OHDA-leasioned striatum to test their survival and function.RESULTS: RT-PCR and immuno-staining demonstrated neuronal/dopaminergic signaling in UCMSCs induced by CPECs-CM and accelerated by addition of retinoic acid (RA) and fibroblast growth factor-2. Expression of b-tubulin-3, Nestin and MAP2 confirmed neuronal differentiation whereas tyrosine hydroxylase, aromatic acid decarboxylase and dopamine transporter were expressed as signs of DOPAMINERGIC differentiation. Post-transplantation, the UCMSCs survived, showed reduced rate of apoptosis and led to animals’ recovery from apomorphine-induced rotations.CONCLUSION: The combination of neurotrophic factors present in CPECs-CM and RA can synergize to maximize DOPAMINERGIC differentiation of potential cell sources including UCMSCs. Our study may have implications for PD cell replacement therapy.

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APA: Copy

ALIAGHAEI, A., GARDANEH, MOSSA, MAGHSOUDI, NADER, SALEHINEJAD, PARVIN, & GHARIB, EHSAN. (2016). DOPAMINERGIC INDUCTION OF UMBILICAL CORD MESENCHYMAL STEM CELLS BY CONDITIONED MEDIUM OF CHOROID PLEXUS EPITHELIAL CELLS REDUCES APOMORPHINE-INDUCED ROTATION IN PARKINSONIAN RATS. ARCHIVES OF IRANIAN MEDICINE, 19(8), 561-570. SID. https://sid.ir/paper/281344/en

Vancouver: Copy

ALIAGHAEI A., GARDANEH MOSSA, MAGHSOUDI NADER, SALEHINEJAD PARVIN, GHARIB EHSAN. DOPAMINERGIC INDUCTION OF UMBILICAL CORD MESENCHYMAL STEM CELLS BY CONDITIONED MEDIUM OF CHOROID PLEXUS EPITHELIAL CELLS REDUCES APOMORPHINE-INDUCED ROTATION IN PARKINSONIAN RATS. ARCHIVES OF IRANIAN MEDICINE[Internet]. 2016;19(8):561-570. Available from: https://sid.ir/paper/281344/en

IEEE: Copy

A. ALIAGHAEI, MOSSA GARDANEH, NADER MAGHSOUDI, PARVIN SALEHINEJAD, and EHSAN GHARIB, “DOPAMINERGIC INDUCTION OF UMBILICAL CORD MESENCHYMAL STEM CELLS BY CONDITIONED MEDIUM OF CHOROID PLEXUS EPITHELIAL CELLS REDUCES APOMORPHINE-INDUCED ROTATION IN PARKINSONIAN RATS,” ARCHIVES OF IRANIAN MEDICINE, vol. 19, no. 8, pp. 561–570, 2016, [Online]. Available: https://sid.ir/paper/281344/en

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