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Information Journal Paper

Title

ASSESSMENT THE EFFICIENCY OF THE CONSTRUCTED MINIGENOME OF RABIES VIRUS USING PV STRAIN AS HELPER VIRUS

Pages

  335-341

Abstract

 INTRODUCTION: Rabies is an acute viral disease that causes encephalomyelitis in mammals and human. The only way to prevent this disease is through vaccination before or after exposure. The aim of this study is to evaluate the efficiency of the Pasteur virus (PV) MINIGENOME, using PV strain.MATERIALS AND METHODS: Enhanced Green Fluorescent Protein (EGFP) sequence was placed between the designed necessary elements (Hammerhead, HDV ribozyme, 3’ Leader, and 5’ Trailer sequences), which resemble the RABIES VIRUS PV strain (PV2061) genome and anti-genome. These constructs were placed between T7 polymerase promoter and T7 polymerase terminator sequences. The accuracy of the MINIGENOME was confirmed by the expression of EGFP using the helper virus in T7-BHK cell line.RESULTS: The viral necessary elements of positive and negative sense strands were evaluated for the ability of EGFP expression in the presence of the helper virus. While the positive strand showed background results, no EGFP background was observed in the negative strand application.CONCLUSION: Establishment of MINIGENOME system does not require advanced biosafety levels. Furthermore, using MINIGENOME system eliminates many potential confounding factors that may be present in coding regions of the genome. Use of the MINIGENOME system is easier and more feasible than the full genome rescue of the virus. This study successfully shows the efficiency of the constructed RABIES VIRUS MINIGENOME in expression of inserted gene.

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    APA: Copy

    AJORLOO, MEHDI, BAMDAD, TARAVAT, GHOLAMI, ALI REZA, & AZADMANESH, KEYHAN. (2016). ASSESSMENT THE EFFICIENCY OF THE CONSTRUCTED MINIGENOME OF RABIES VIRUS USING PV STRAIN AS HELPER VIRUS. ARCHIVES OF IRANIAN MEDICINE, 19(5), 335-341. SID. https://sid.ir/paper/281421/en

    Vancouver: Copy

    AJORLOO MEHDI, BAMDAD TARAVAT, GHOLAMI ALI REZA, AZADMANESH KEYHAN. ASSESSMENT THE EFFICIENCY OF THE CONSTRUCTED MINIGENOME OF RABIES VIRUS USING PV STRAIN AS HELPER VIRUS. ARCHIVES OF IRANIAN MEDICINE[Internet]. 2016;19(5):335-341. Available from: https://sid.ir/paper/281421/en

    IEEE: Copy

    MEHDI AJORLOO, TARAVAT BAMDAD, ALI REZA GHOLAMI, and KEYHAN AZADMANESH, “ASSESSMENT THE EFFICIENCY OF THE CONSTRUCTED MINIGENOME OF RABIES VIRUS USING PV STRAIN AS HELPER VIRUS,” ARCHIVES OF IRANIAN MEDICINE, vol. 19, no. 5, pp. 335–341, 2016, [Online]. Available: https://sid.ir/paper/281421/en

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