EFFECT OF PREGNANT MOUSE SERUM ON INDUCTION OF INDOLAMINE 2, 3- DIOXYGENASE IN DENDRITIC CELLS

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NIKOU SH.; MOAZENI S.M.; BOZORG MEHR M.; ZARNANI A.H.

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Journal: Journal of Reproduction & Infertility Year:2006 | Volume:7 | Issue:3 (28) Page(s): 187-197

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Title

EFFECT OF PREGNANT MOUSE SERUM ON INDUCTION OF INDOLAMINE 2, 3- DIOXYGENASE IN DENDRITIC CELLS

Author(s)

NIKOU SH. | MOAZENI S.M. | BOZORG MEHR M. | ZARNANI A.H. | Issue Writer Certificate

Keywords

PREGNANCYQ2

DENDRITIC CELLSQ3

IDOQ2

ALLOGENIC MLRQ2

HPLCQ2

Abstract

Introduction: Several studies have shown that many factors are involved in the maternal tolerance to the fetus. Indolamine 2, 3- dioxygenase (IDO) enzyme which catabolizes tryptophan is one of the factors that have been reported to play an important role leading to a successful PREGNANCY. The objective of this study was to evaluate the effects of pregnant mouse serum on the induction of indolamine 2, 3 dioxygenase in DENDRITIC CELLS (DCs) which may be used as a basis for practical studies on the immunological bases of recurrent abortions.Materials & Methods: Allogenic pregnant mice sera were collected in mid-PREGNANCY. DCs were isolated from Balb/c mouse spleen through a three-step method, including: Collagenase digestion of spleen tissue, low density cells separation via the Nycodenz density gradient centrifugation and plastic adherence. T cells were isolated from C57BL/6 mouse lymph nodes through nylon wool method. As stimulator cells, pregnant and non-pregnant mice sera treated DCs were irradiated and co-cultured with purified T cells (allogenic MLR). 1-methyl-tryptophan (1-MT), as the specific inhibitor of IDO, was added to some wells of MLR assay in different concentrations and T cells proliferation response was measured by H-Thymidine incorporation. The MLR supernatant was also analyzed by HPLC for its tryptophan and kynurenin (Trp metabolite) content. All tests were repeated for 5 times. Man-Whitney's non- parametric test was used to evaluate the differences among groups. Confidence interval was 95% and p-values <0.05 were regarded as significant.Results: The results showed the ability of pregnant mice sera to reduce the DENDRITIC CELLS ability in T cell proliferation induction compared to non-pregnant mice sera but addition of 1-MT did not have any significant effect on this inhibition. Additionally, IDO metabolites concentration assessment in the presence or absence of 1-MT, through HPLC method, did not show any significant difference.Conclusion: There are many factors in pregnant mice sera such as progesterone, IL-10, Vit D3, etc. Which might cause inhibition of T lymphocyte proliferation response in ALLOGENIC MLR through affecting DCs' efficiency? Although it seems that IDO expression by DCs is not responsible for decrease in T cell proliferation after treatment of DCs by pregnant mice sera, thus some other mechanisms might be responsible for this phenomenon which their identification needs more investigation.

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APA: Copy

NIKOU, SH., MOAZENI, S.M., BOZORG MEHR, M., & ZARNANI, A.H.. (2006). EFFECT OF PREGNANT MOUSE SERUM ON INDUCTION OF INDOLAMINE 2, 3- DIOXYGENASE IN DENDRITIC CELLS. JOURNAL OF REPRODUCTION AND INFERTILITY, 7(3 (28)), 187-197. SID. https://sid.ir/paper/28502/en

Vancouver: Copy

NIKOU SH., MOAZENI S.M., BOZORG MEHR M., ZARNANI A.H.. EFFECT OF PREGNANT MOUSE SERUM ON INDUCTION OF INDOLAMINE 2, 3- DIOXYGENASE IN DENDRITIC CELLS. JOURNAL OF REPRODUCTION AND INFERTILITY[Internet]. 2006;7(3 (28)):187-197. Available from: https://sid.ir/paper/28502/en

IEEE: Copy

SH. NIKOU, S.M. MOAZENI, M. BOZORG MEHR, and A.H. ZARNANI, “EFFECT OF PREGNANT MOUSE SERUM ON INDUCTION OF INDOLAMINE 2, 3- DIOXYGENASE IN DENDRITIC CELLS,” JOURNAL OF REPRODUCTION AND INFERTILITY, vol. 7, no. 3 (28), pp. 187–197, 2006, [Online]. Available: https://sid.ir/paper/28502/en

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