A KINETIC COMPARISON ON THE INHIBITION OF ADENOSINE DEAMINASE BY PURINE DRUGS

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ATAEI GH.; BAGHERI SOGHRA; DIVSALAR A.; SABOURI A.A.; SAFARIAN SH.; NAMAKI S.; MOUSAVI MOVAHEDI ALI AKBAR

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Journal Paper

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Journal: Iranian Journal of Pharmaceutical Research Year:2007 | Volume:6 | Issue:1 Page(s): 43-50

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Title

A KINETIC COMPARISON ON THE INHIBITION OF ADENOSINE DEAMINASE BY PURINE DRUGS

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Keywords

ADENOSINE DEAMINASE

ALLOPURINOL

ACYCLOVIR

THEOPHYLLINE

KINETIC

Abstract

The effects of ALLOPURINOL, ACYCLOVIR and THEOPHYLLINE on the activity of ADENOSINE DEAMINASE (ADA) were studied in 50mM sodium phosphate buffer pH 7.5 at 27oC, using a UV–Vis spectrophotometer. ADENOSINE DEAMINASE is inhibited by these ligands, via different types of inhibition. ALLOPURINOL, as a transition state analog of xanthine oxidase, and ACYCLOVIR competitively inhibit the catalytic activity of ADA. Inhibition constant values are 285 and 231mM for ALLOPURINOL and ACYCLOVIR, respectively. THEOPHYLLINE acts as a non-competitive inhibitor for ADA, which shows different affinity binding sites at various drug concentrations. There were two different types of inhibition constant, one of them due to a low concentration of the drug (Ki=56mM) and the other appearing at higher concentrations of THEOPHYLLINE (Ki=201mM). Thermodynamic parameters also show that ADA has two binding sites for THEOPHYLLINE. The comparison of inhibition constant for inosine (Ki=143mM) and ACYCLOVIR (Ki=231mM) elucidates the critical role of the ribose ring within the inosine structure, relative to the open ring of ACYCLOVIR. Comparison of the inhibition constant of theobromine (Ki=311mM) with inosine (Ki=143mM) shows the critical binding role of N7 position within the purine ring. Interestingly, the N7 position in ALLOPURINOL is replaced by a CH2 group, which demonstrates the lower inhibiting potency of ALLOPURINOL (Ki=285mM) relative to inosine (Ki=143mM). In a structural sense, a comparison made between the structure of THEOPHYLLINE and theobromine besides a comparison between the inhibition constant of THEOPHYLLINE (Ki=56mM at low and 201mM at higher concentrations) and caffeine (Ki=342mM) indicate that substitution of a bulky group in N1 and N7 positions of purine has a critical role in the binding affinity of the above- mentioned inhibitors to the enzyme.

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APA: Copy

ATAEI, GH., BAGHERI, SOGHRA, SABOURI, A.A., SAFARIAN, SH., MOUSAVI MOVAHEDI, ALI AKBAR, DIVSALAR, A., & NAMAKI, S.. (2007). A KINETIC COMPARISON ON THE INHIBITION OF ADENOSINE DEAMINASE BY PURINE DRUGS . IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 6(1), 43-50. SID. https://sid.ir/paper/287613/en

Vancouver: Copy

ATAEI GH., BAGHERI SOGHRA, SABOURI A.A., SAFARIAN SH., MOUSAVI MOVAHEDI ALI AKBAR, DIVSALAR A., NAMAKI S.. A KINETIC COMPARISON ON THE INHIBITION OF ADENOSINE DEAMINASE BY PURINE DRUGS . IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2007;6(1):43-50. Available from: https://sid.ir/paper/287613/en

IEEE: Copy

GH. ATAEI, SOGHRA BAGHERI, A.A. SABOURI, SH. SAFARIAN, ALI AKBAR MOUSAVI MOVAHEDI, A. DIVSALAR, and S. NAMAKI, “A KINETIC COMPARISON ON THE INHIBITION OF ADENOSINE DEAMINASE BY PURINE DRUGS ,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 6, no. 1, pp. 43–50, 2007, [Online]. Available: https://sid.ir/paper/287613/en

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