EVALUATION OF THE EFFECT OF PEGYLATED SINGLE-WALLED CARBON NANOTUBES ON VIABILITY AND PROLIFERATION OF JURKAT CELLS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HADIDI NAGHMEH; HOSSEINI SHIRAZI SEYED FARSHAD; KOBARFARD FARZAD; NAFISSI VARCHEH NASTARAN; ABOOFAZELI REZA

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Pharmaceutical Research Year:2012 | Volume:11 | Issue:1 Page(s): 27-37

Download Full-Text

View:

264

Download:

Cites:

Information Journal Paper

Title

EVALUATION OF THE EFFECT OF PEGYLATED SINGLE-WALLED CARBON NANOTUBES ON VIABILITY AND PROLIFERATION OF JURKAT CELLS

Author(s)

Keywords

PHOSPHOLIPID-PEG

CYTOTOXICITY

FLOW CYTOMETRY

FUNCTIONALIZATION

CELL CULTURE

Abstract

Among the numerous nanosized drug delivery systems currently under investigation, carbon nanotubes (CNTs), regardless of being single or multiple-walled, offer several advantages and are considered as promising candidates for drug targeting. Despite the valuable potentials of CNTs in drug delivery, their toxicity still remains an important issue. After the PEGylation of single-walled CNTs (SWCNTs) with PHOSPHOLIPID-PEG (Pl-PEG) conjugates to prepare water-dispersible nanostructures, the present study was designed to evaluate whether the FUNCTIONALIZATION with Pl-PEG derivatives could alter the cytotoxic response of cells in culture, affect their viability and proliferation. In-vitro CYTOTOXICITY screens were performed on cultured Jurkat cells. The SWCNTs samples used in this exposure were pristine SWCNTs, Pl-PEG 2000/5000-SWCNTs at various concentrations. Jurkat cells were first incubated for 3 h at 37oC with test materials and seeded in 6-well culture plates at a given concentration. The plates were then incubated for 24, 48 and 72 h at 37oC in a 5% CO2 humidified incubator. Cell Viability and proliferation assay were performed using trypan blue exclusion test and the cell cycle kinetic status of Jurkat cells was analyzed by FLOW CYTOMETRY. Cell morphology was finally studied using double staining technique and a fluorescence microscope. We found that, regardless of the duration of exposure, functionalized SWCNTs were substantially less toxic, compared to pure SWCNTs and that the molecular weight of Pl-PEGs played an important role at higher concentrations. In conclusion, our noncovalent protocol seemed to be effective for increasing SWCNTs biocompatibility.

Cites

No record.

References

No record.

Cite

APA: Copy

HADIDI, NAGHMEH, HOSSEINI SHIRAZI, SEYED FARSHAD, KOBARFARD, FARZAD, NAFISSI VARCHEH, NASTARAN, & ABOOFAZELI, REZA. (2012). EVALUATION OF THE EFFECT OF PEGYLATED SINGLE-WALLED CARBON NANOTUBES ON VIABILITY AND PROLIFERATION OF JURKAT CELLS. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 11(1), 27-37. SID. https://sid.ir/paper/287939/en

Vancouver: Copy

HADIDI NAGHMEH, HOSSEINI SHIRAZI SEYED FARSHAD, KOBARFARD FARZAD, NAFISSI VARCHEH NASTARAN, ABOOFAZELI REZA. EVALUATION OF THE EFFECT OF PEGYLATED SINGLE-WALLED CARBON NANOTUBES ON VIABILITY AND PROLIFERATION OF JURKAT CELLS. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2012;11(1):27-37. Available from: https://sid.ir/paper/287939/en

IEEE: Copy

NAGHMEH HADIDI, SEYED FARSHAD HOSSEINI SHIRAZI, FARZAD KOBARFARD, NASTARAN NAFISSI VARCHEH, and REZA ABOOFAZELI, “EVALUATION OF THE EFFECT OF PEGYLATED SINGLE-WALLED CARBON NANOTUBES ON VIABILITY AND PROLIFERATION OF JURKAT CELLS,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 11, no. 1, pp. 27–37, 2012, [Online]. Available: https://sid.ir/paper/287939/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops