THE TIME COURSE OF JNK AND P38 ACTIVATION IN CEREBELLAR GRANULE NEURONS FOLLOWING GLUCOSE DEPRIVATION AND BDNF TREATMENT

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

VAKILI ZAHIR NIKI; ABKHEZR MOUSA; KHAJE PIRI ZAHRA; OSTAD SEYYED NASSER; KEBRIAEZADE ABBASS; GHAHREMANI MOHAMMAD HOSSEIN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Pharmaceutical Research Year:2012 | Volume:11 | Issue:1 Page(s): 315-323

Download Full-Text

View:

340

Download:

Cites:

Information Journal Paper

Title

THE TIME COURSE OF JNK AND P38 ACTIVATION IN CEREBELLAR GRANULE NEURONS FOLLOWING GLUCOSE DEPRIVATION AND BDNF TREATMENT

Author(s)

Keywords

MAPKS

CGN

BRAIN-DERIVED NEUROTROPHIC FACTOR

SIGNALING

Abstract

Low glucose condition induces neuronal cell-death via intracellular mechanisms including mitogen-activated protein kinases (MAPK) SIGNALING pathways. It has been shown that low glucose medium decreases neuronal survival in cerebellar granule neurons (CGNs). In this study, we have examined the activation of JNK, p38kinase and ERK1/2 pathways in low glucose medium in CGNs.The CGNs were prepared from new-born (P-2 and P-5) rats and cultured in Dulbecco′s Modified Eagle′s Medium high (DMEM-HIGH) glucose supplemented with Fetal Bovine Serum (FBS) 10% for 7 days. The glucose deprivation was induced through replacing theculture medium with the low glucose (5 mM) medium. The MAPK pathways activation was evaluated through phospho specific antibodies using western blot. The viability of cells was measuring using MTT assay.The results indicated that low glucose reduces the cell survival and BRAIN-DERIVED NEUROTROPHIC FACTOR (BDNF) elevates the cell viability in CGNs. The basal c-Jun N-terminal kinase (JNK) activity was high in CGNs and glucose deprivation for 24 h had increased phospho-JNK level to 2-fold compared to basal. BDNF treatment reduced the basal JNK activity within 30 min but had no effect in longer incubations. BDNF also blocked the low glucose-induced JNK activation. In addition, CGNs exhibited high p38 phosphorylation in low glucose medium in 48 h.These results demonstrated that in sustained low glucose conditions, CGNs had high activityof stress-activated MAPK which could induce cellular damage. Moreover, BDNF can prevent JNK and p38 activation in stress conditions and increase cell viability. Our results suggest that in sustained stress conditions, inhibition of JNK and/or p38 pathways might protect neurons from damage in low glucose conditions.

Cites

No record.

References

No record.

Cite

APA: Copy

VAKILI ZAHIR, NIKI, ABKHEZR, MOUSA, KHAJE PIRI, ZAHRA, OSTAD, SEYYED NASSER, KEBRIAEZADE, ABBASS, & GHAHREMANI, MOHAMMAD HOSSEIN. (2012). THE TIME COURSE OF JNK AND P38 ACTIVATION IN CEREBELLAR GRANULE NEURONS FOLLOWING GLUCOSE DEPRIVATION AND BDNF TREATMENT. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 11(1), 315-323. SID. https://sid.ir/paper/287941/en

Vancouver: Copy

VAKILI ZAHIR NIKI, ABKHEZR MOUSA, KHAJE PIRI ZAHRA, OSTAD SEYYED NASSER, KEBRIAEZADE ABBASS, GHAHREMANI MOHAMMAD HOSSEIN. THE TIME COURSE OF JNK AND P38 ACTIVATION IN CEREBELLAR GRANULE NEURONS FOLLOWING GLUCOSE DEPRIVATION AND BDNF TREATMENT. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2012;11(1):315-323. Available from: https://sid.ir/paper/287941/en

IEEE: Copy

NIKI VAKILI ZAHIR, MOUSA ABKHEZR, ZAHRA KHAJE PIRI, SEYYED NASSER OSTAD, ABBASS KEBRIAEZADE, and MOHAMMAD HOSSEIN GHAHREMANI, “THE TIME COURSE OF JNK AND P38 ACTIVATION IN CEREBELLAR GRANULE NEURONS FOLLOWING GLUCOSE DEPRIVATION AND BDNF TREATMENT,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 11, no. 1, pp. 315–323, 2012, [Online]. Available: https://sid.ir/paper/287941/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops