TWO BIOLOGICAL ACTIVE FRACTIONS ISOLATED FROM BUTHOTUS SCHACH (BS) SCORPION VENOM EXAMINED ON STRIATED MUSCLE PREPARATION, IN-VITRO

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VATANPOUR HOSSEIN; AHMADI FARHAD; ZARE MIRAKABADI ABBAS; JALALI AMIR

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Journal Paper

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Journal: Iranian Journal of Pharmaceutical Research Year:2012 | Volume:11 | Issue:3 Page(s): 905-911

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Title

TWO BIOLOGICAL ACTIVE FRACTIONS ISOLATED FROM BUTHOTUS SCHACH (BS) SCORPION VENOM EXAMINED ON STRIATED MUSCLE PREPARATION, IN-VITRO

Author(s)

VATANPOUR HOSSEIN | AHMADI FARHAD | ZARE MIRAKABADI ABBAS | JALALI AMIR | Issue Writer Certificate

Keywords

TWITCH

PURIFICATION

CRUDE VENOM

CONTRACTURE

BUTHOTUS SCHACH

Abstract

Buthotus schachis one of the most dangerous scorpions in tropical part of Iran. The effects of its CRUDE VENOM at 1, 3, 10 mg/mL and its obtained fractions by gel filtrations were investigated on neuromuscular transmission. CBC and MHD indirectly and directly stimulated preparations techniques were used to study their possible pre or post junctional activities. At 3 and 10 mg/mL (not at 1 mg/mL), BS venom caused initiall increase in TWITCH height followed by blockage due to large contraction that responded gradually at the same time. CONTRACTURE responses to exogenous Ach (1-2 mM, 30 sec) and Carb (30-40 mM, 60 sec) in the presence of the venom were not increased which does show no anticholinstrease effects. Furthermore CONTRACTURE response to KCl (20-40 mM, 30 sec) does changed exposure to venom in CBC preparations. On the other hand the effects of the venom in response to directly stimulated preparations was shallower than in indirect stimulated preparations. So in agreement with KCL response BS venom affects mostly prejunctionally to facilitate the neurotransmitter release rather than postjunctionally effects. To access bioactive components, seven fractions were collected by gel filtrations techniques. Among the fractions F6, LD50=21 mg<F4, LD50=35.5 mg<Venom LD50=84 mg per mice were more toxic respectively. Both fractions show the same effects but stronger than venom on TWITCH height responses in indirectly stimulated CBC preparations.Finally, according to our results venom as well as fractions F4 and F6 act mostly prejunctionally on Ach release. More attempt is carrying out to study their effects on ion channel activities.

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APA: Copy

VATANPOUR, HOSSEIN, AHMADI, FARHAD, ZARE MIRAKABADI, ABBAS, & JALALI, AMIR. (2012). TWO BIOLOGICAL ACTIVE FRACTIONS ISOLATED FROM BUTHOTUS SCHACH (BS) SCORPION VENOM EXAMINED ON STRIATED MUSCLE PREPARATION, IN-VITRO. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 11(3), 905-911. SID. https://sid.ir/paper/287985/en

Vancouver: Copy

VATANPOUR HOSSEIN, AHMADI FARHAD, ZARE MIRAKABADI ABBAS, JALALI AMIR. TWO BIOLOGICAL ACTIVE FRACTIONS ISOLATED FROM BUTHOTUS SCHACH (BS) SCORPION VENOM EXAMINED ON STRIATED MUSCLE PREPARATION, IN-VITRO. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2012;11(3):905-911. Available from: https://sid.ir/paper/287985/en

IEEE: Copy

HOSSEIN VATANPOUR, FARHAD AHMADI, ABBAS ZARE MIRAKABADI, and AMIR JALALI, “TWO BIOLOGICAL ACTIVE FRACTIONS ISOLATED FROM BUTHOTUS SCHACH (BS) SCORPION VENOM EXAMINED ON STRIATED MUSCLE PREPARATION, IN-VITRO,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 11, no. 3, pp. 905–911, 2012, [Online]. Available: https://sid.ir/paper/287985/en

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