A REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR DETERMINATION OF RAPAMYCIN

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SOBHANI HAMIDEH; SHAFAATI ALIREZA; NAFISSI VARCHEH NASTARAN; ABOOFAZELI REZA

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Journal Paper

Paper Information

Journal: Iranian Journal of Pharmaceutical Research Year:2013 | Volume:12 | Issue:SUPPLEMENT 1 Page(s): 77-81

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Title

A REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR DETERMINATION OF RAPAMYCIN

Author(s)

SOBHANI HAMIDEH | SHAFAATI ALIREZA | NAFISSI VARCHEH NASTARAN | ABOOFAZELI REZA | Issue Writer Certificate

Keywords

RAPAMYCIN

SIROLIMUS

RP- HPLC

UV DETECTION

Abstract

Easily degradating and various isomeric forms of RAPAMYCIN (Sirolimus) face the determination of this compound to many challenges. In this study, we developed and validated the isocratic reversed phase high performance liquid chromatographic (RP-HPLC) method for RAPAMYCIN. Separation was performed on a C8 column (MZ, 15´4.6 mm, 5 mm particle size) using methanol: water (80: 20 v/v) as the mobile phase with the flow rate of 1 mL/min. The column temperature was set at 57oC and the detection was carried out at the wavelength of 277 nm. The method was linear over a concentration range of 0.025-2 mg/mL. The coefficient of variation of intra- and inter-day, assessed at three concentration levels of 0.075, 0.3 and 0.900 mg/mL, was less than 2%. Limit of quantification (LOQ) was found 25 ng/mL. The method with high percent recovery and short retention time of RAPAMYCIN, was found to be simple, rapid and reproducible.

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APA: Copy

SOBHANI, HAMIDEH, SHAFAATI, ALIREZA, NAFISSI VARCHEH, NASTARAN, & ABOOFAZELI, REZA. (2013). A REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR DETERMINATION OF RAPAMYCIN. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 12(SUPPLEMENT 1), 77-81. SID. https://sid.ir/paper/288057/en

Vancouver: Copy

SOBHANI HAMIDEH, SHAFAATI ALIREZA, NAFISSI VARCHEH NASTARAN, ABOOFAZELI REZA. A REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR DETERMINATION OF RAPAMYCIN. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2013;12(SUPPLEMENT 1):77-81. Available from: https://sid.ir/paper/288057/en

IEEE: Copy

HAMIDEH SOBHANI, ALIREZA SHAFAATI, NASTARAN NAFISSI VARCHEH, and REZA ABOOFAZELI, “A REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR DETERMINATION OF RAPAMYCIN,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 12, no. SUPPLEMENT 1, pp. 77–81, 2013, [Online]. Available: https://sid.ir/paper/288057/en

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