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Information Journal Paper

Title

STUDYING THE STABILITY OF S-LAYER PROTEIN OF LACTOBACILLUS ACIDOPHILUS ATCC 4356 IN SIMULATED GASTROINTESTINAL FLUIDS USING SDS-PAGE AND CIRCULAR DICHROISM

Pages

  47-56

Abstract

 Crystalline arrays of proteinaceous subunits forming surface layers (S-layers) are now recognized as one of the most common outermost cell envelope components of prokaryotic organisms. The surface layer protein of LACTOBACILLUS ACIDOPHILUS ATCC4356 is composed of a single species of protein of apparent molecular weight of 43-46 KDa. Considering the LACTOBACILLUS ACIDOPHILUS ATCC4356 having the S-layer is stable in harsh gastrointestinal (GI) conditions, a protective role against destructive GI factors which has been proposed for these nanostructures. It opens interesting perspectives in the using and development of this S-layer as a protective coat for oral administration of unstable drug nanocarriers. To achieve this goal, it is necessary to study the in-vitro STABILITY of the S-layers in the simulated gastrointestinal fluids (SGIF). This study was planned to evaluate the in-vitro STABILITY of the extracted S-LAYER PROTEIN of LACTOBACILLUS ACIDOPHILUS ATCC4356 in SGIF using it as a protective coat in oral drug delivery. Sodium dodecyl sulfate GEL ELECTROPHORESIS (SDS-PAGE) and CIRCULAR DICHROISM (CD) spectroscopy were used to study the STABILITY of the S-LAYER PROTEIN incubated in SGIF. Both the SDS-PAGE and CD spectra results showed that LACTOBACILLUS ACIDOPHILUS ATCC4356 S-LAYER PROTEIN is stable in simulated gastric fluid (SGF) with pH=2 up to 5 min. It is stable in SGF pH=3.2 and above it, with and without pepsin. It is also stable in all the simulated intestinal fluids. This S-layer is also stable in all of the simulated intestinal fluids.

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    • Cite

    APA: Copy

    ESLAMI, NEDA, KASRA KERMANSHAHI, ROOHA, & ERFAN, MOHAMMAD. (2013). STUDYING THE STABILITY OF S-LAYER PROTEIN OF LACTOBACILLUS ACIDOPHILUS ATCC 4356 IN SIMULATED GASTROINTESTINAL FLUIDS USING SDS-PAGE AND CIRCULAR DICHROISM. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 12(SUPPLEMENT 1), 47-56. SID. https://sid.ir/paper/288066/en

    Vancouver: Copy

    ESLAMI NEDA, KASRA KERMANSHAHI ROOHA, ERFAN MOHAMMAD. STUDYING THE STABILITY OF S-LAYER PROTEIN OF LACTOBACILLUS ACIDOPHILUS ATCC 4356 IN SIMULATED GASTROINTESTINAL FLUIDS USING SDS-PAGE AND CIRCULAR DICHROISM. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2013;12(SUPPLEMENT 1):47-56. Available from: https://sid.ir/paper/288066/en

    IEEE: Copy

    NEDA ESLAMI, ROOHA KASRA KERMANSHAHI, and MOHAMMAD ERFAN, “STUDYING THE STABILITY OF S-LAYER PROTEIN OF LACTOBACILLUS ACIDOPHILUS ATCC 4356 IN SIMULATED GASTROINTESTINAL FLUIDS USING SDS-PAGE AND CIRCULAR DICHROISM,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 12, no. SUPPLEMENT 1, pp. 47–56, 2013, [Online]. Available: https://sid.ir/paper/288066/en

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