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Information Journal Paper

Title

THE EFFECT OF PENTOXIFYLLINE ON BCL-2 GENE EXPRESSION CHANGES IN HIPPOCAMPUS AFTER ISCHEMIA-REPERFUSION IN WISTAR RATS BY A QUATITATIVE RT-PCR METHOD

Pages

  495-501

Abstract

 ISCHEMIA-REPERFUSION injury is the tissue damage caused when blood supply returns to the tissue after a period of ischemia or lack of oxygen. ISCHEMIA-REPERFUSION brain injury initiates an inflammatory response involving the expression of adhesion molecules and cytokines. Twenty-four male Wistar rats (250-300 g body wt) were used in this study. The animals were divided into four groups of 6 rats each: I: Control group that was subjected to ischemiareperfusion, II: ISCHEMIA-REPERFUSION group that was subjected to all surgical procedures, III: Drug group that received pentoxifylline (200, 400 and 600 mg/kg) 60 min before and after ischemia and IV: Vehicle group that received saline. Seventy two h after ISCHEMIA-REPERFUSION, the hippocampus was taken for studying the changes in BCL-2 gene expression. We used quantitative REAL-TIME PCR for the detection of BCL-2 gene expression in ischemia and drug groups and then compared them to normal samples. The results showed the gene dosage ratio of 0.66 and 1.5 for ischemia group and the drug groups, respectively. The results also showed the BCL-2 gene expression declined in ischemia group as compared to the drug group. Furthermore, we observed a significant difference in the BCL-2 gene expression between ischemia and drug groups. These findings are consistent with anti-apoptotic properties of BCL-2 gene. Furthermore this method provides a powerful tool for the investigators to study brain ischemia and respond to the treatment drugs with anti-apoptotic agents.

Cites

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    • Cite

    APA: Copy

    SARI, SOYAR, HASHEMI, MEHRDAD, MAHDIAN, REZA, PARIVAR, KAZEM, & REZAYAT, MAHDI. (2013). THE EFFECT OF PENTOXIFYLLINE ON BCL-2 GENE EXPRESSION CHANGES IN HIPPOCAMPUS AFTER ISCHEMIA-REPERFUSION IN WISTAR RATS BY A QUATITATIVE RT-PCR METHOD. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 12(3), 495-501. SID. https://sid.ir/paper/288116/en

    Vancouver: Copy

    SARI SOYAR, HASHEMI MEHRDAD, MAHDIAN REZA, PARIVAR KAZEM, REZAYAT MAHDI. THE EFFECT OF PENTOXIFYLLINE ON BCL-2 GENE EXPRESSION CHANGES IN HIPPOCAMPUS AFTER ISCHEMIA-REPERFUSION IN WISTAR RATS BY A QUATITATIVE RT-PCR METHOD. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2013;12(3):495-501. Available from: https://sid.ir/paper/288116/en

    IEEE: Copy

    SOYAR SARI, MEHRDAD HASHEMI, REZA MAHDIAN, KAZEM PARIVAR, and MAHDI REZAYAT, “THE EFFECT OF PENTOXIFYLLINE ON BCL-2 GENE EXPRESSION CHANGES IN HIPPOCAMPUS AFTER ISCHEMIA-REPERFUSION IN WISTAR RATS BY A QUATITATIVE RT-PCR METHOD,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 12, no. 3, pp. 495–501, 2013, [Online]. Available: https://sid.ir/paper/288116/en

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