THE EFFECT OF PAXILLINE ON EARLY ALTERATIONS OF ELECTROPHYSIOLOGICAL PROPERTIES OF DENTATE GYRUS GRANULE CELLS IN PILOCARPINE-TREATED RATS

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MEHRANFARD NASRIN; GHOLAMIPOUR BADIE HAMID; MOTAMEDI FERESHTEH; JANAHMADI MAHYAR; NADERI NIMA

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Journal: Iranian Journal of Pharmaceutical Research Year:2014 | Volume:13 | Issue:SUPPL Page(s): 125-132

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Title

THE EFFECT OF PAXILLINE ON EARLY ALTERATIONS OF ELECTROPHYSIOLOGICAL PROPERTIES OF DENTATE GYRUS GRANULE CELLS IN PILOCARPINE-TREATED RATS

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Keywords

PAXILLINE

DENTATE GYRUS

GRANULE CELLS

EPILEPSY

Abstract

The DENTATE GYRUS of hippocampus has long been considered as a focal point for studies on mechanisms responsible for the development of temporal lobe EPILEPSY (TLE). Change in intrinsic properties of DENTATE GYRUS GRANULE CELLS (GCs) has been considered as an important factor responsible in temporal lobe seizures. In this study, we evaluated the intrinsic properties of GCs, during acute phase of seizure (24 h after i.p. injection of pilocarpine) compared to sham group using whole cell patch-clamp recordings. Our results showed a significant increase in the number of action potentials (APs) after applying depolarizing currents of 200 pA (p<0.01) and 250pA (p<0.05) compared to sham group. The evaluation of AP properties revealed a decrease in half-width of AP in GCs of seizure group (1.27±0.03 ms) compared to sham group (1.60±0.11). Moreover, addition of BAPTA to pipette solution prevented changes in AP half-width in seizure group (1.71±0.11 ms) compared to sham group (1.91±0.08 ms). In contrast, an increase in the amplitude of fast afterhyperpolarization was observed in GCs of seizure group (-11.68±0.72 mV) compared to sham group (-8.28±0.59 mV). Also, GCs of seizure group showed a significant increase in both firing rate and instantaneous firing frequency at depolarizing currents of 200 pA (P<0.01) and 250 pA (P<0.05) compared to sham group. The changes in electrophysiological properties of GCs were attenuated after bath application of PAXILLINE suggesting possible involvement of large conductance Ca2+- activated K+channel (BK channel). Our results suggested the possible involvement of certain potassium channels in early changes of intrinsic properties of GCs which eventually facilitate TLE development.

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APA: Copy

MEHRANFARD, NASRIN, GHOLAMIPOUR BADIE, HAMID, MOTAMEDI, FERESHTEH, JANAHMADI, MAHYAR, & NADERI, NIMA. (2014). THE EFFECT OF PAXILLINE ON EARLY ALTERATIONS OF ELECTROPHYSIOLOGICAL PROPERTIES OF DENTATE GYRUS GRANULE CELLS IN PILOCARPINE-TREATED RATS. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 13(SUPPL), 125-132. SID. https://sid.ir/paper/288429/en

Vancouver: Copy

MEHRANFARD NASRIN, GHOLAMIPOUR BADIE HAMID, MOTAMEDI FERESHTEH, JANAHMADI MAHYAR, NADERI NIMA. THE EFFECT OF PAXILLINE ON EARLY ALTERATIONS OF ELECTROPHYSIOLOGICAL PROPERTIES OF DENTATE GYRUS GRANULE CELLS IN PILOCARPINE-TREATED RATS. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2014;13(SUPPL):125-132. Available from: https://sid.ir/paper/288429/en

IEEE: Copy

NASRIN MEHRANFARD, HAMID GHOLAMIPOUR BADIE, FERESHTEH MOTAMEDI, MAHYAR JANAHMADI, and NIMA NADERI, “THE EFFECT OF PAXILLINE ON EARLY ALTERATIONS OF ELECTROPHYSIOLOGICAL PROPERTIES OF DENTATE GYRUS GRANULE CELLS IN PILOCARPINE-TREATED RATS,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 13, no. SUPPL, pp. 125–132, 2014, [Online]. Available: https://sid.ir/paper/288429/en

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