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Information Journal Paper

Title

Potent Antioxidant Properties of rolB-transformed Catharanthus roseus (L. ) G. Don

Pages

  537-550

Abstract

 Free radicals play an important role in pathological processes such as aging and developing cancer; hence, natural products inhibit free radical production, and can play an important role in preventing diseases. We aimed to evaluate the scavenging activity of free radicals, reducing power, inhibition of lipid peroxidation and malondialdehyde (MDA) formation, and the antioxidant composition of rolB-Transformed hairy roots and Leaf callus of Catharanthus roseus. Hairy roots of the Catharanthus roseus were induced using Agrobacterium rhizogenes strain ATCC 15834 to transfer the rolB gene. Polymerase chain reaction analysis was used to identify the presence of the gene in the Transformed hairy roots. Folin-Ciocalteu reagent, aluminum chloride calorimetry and high performance liquid chromatography were used to determine the content of total phenolic, flavonoid and gallic acid, respectively. To this end, we assayed the free radicals scavenging activity by 1-diphenyl-2-picrylhydrazyl (DPPH), reducing power, inhibition of lipid peroxidation and inhibition of malondialdehyde production. The results showed that phenolic, flavonoid, and gallic acid contents in the ethanol extract of the hairy roots were signifi cantly higher (p ≤ 0. 01) than those naturally found in the extracts of root, leaf, and Leaf callus of C. roseus. The hairy roots extract showed the lowest IC50 for the inhibition of DPPH• . Furthermore, the ethanol extract showed the best reducing power and had the highest potential to inhibit lipid peroxidation and to form the MDA. The Transformed hairy roots can be considered a rich natural source of antioxidants.

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    APA: Copy

    MARDANINEJAD, SHAHIN, KHAVARI NEJAD, RAMAZAN ALI, SAADATMAND, SARA, NAJAFI, FARZANEH, & ABEROOMAND AZAR, PARVIZ. (2016). Potent Antioxidant Properties of rolB-transformed Catharanthus roseus (L. ) G. Don. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 15(2), 537-550. SID. https://sid.ir/paper/288642/en

    Vancouver: Copy

    MARDANINEJAD SHAHIN, KHAVARI NEJAD RAMAZAN ALI, SAADATMAND SARA, NAJAFI FARZANEH, ABEROOMAND AZAR PARVIZ. Potent Antioxidant Properties of rolB-transformed Catharanthus roseus (L. ) G. Don. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2016;15(2):537-550. Available from: https://sid.ir/paper/288642/en

    IEEE: Copy

    SHAHIN MARDANINEJAD, RAMAZAN ALI KHAVARI NEJAD, SARA SAADATMAND, FARZANEH NAJAFI, and PARVIZ ABEROOMAND AZAR, “Potent Antioxidant Properties of rolB-transformed Catharanthus roseus (L. ) G. Don,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 15, no. 2, pp. 537–550, 2016, [Online]. Available: https://sid.ir/paper/288642/en

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