SCREENING FOR TYPE II L-ASPARAGINASES: LESSONS FROM THE GENUS HALOMONAS

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SHARAFI ZEINAB; BARATI MAHMOOD; KHOSHAYAND MOHAMMAD REZA; ADRANGI SINA

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Journal Paper

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Journal: Iranian Journal of Pharmaceutical Research Year:2017 | Volume:16 | Issue:4 Page(s): 1565-1573

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Title

SCREENING FOR TYPE II L-ASPARAGINASES: LESSONS FROM THE GENUS HALOMONAS

Author(s)

SHARAFI ZEINAB | BARATI MAHMOOD | KHOSHAYAND MOHAMMAD REZA | ADRANGI SINA | Issue Writer Certificate

Keywords

SCREENING

L-ASPARAGINASE

HALOMONAS

OLIGOTROPHY

OPTIMIZATION

Abstract

Among the two types of bacterial L-ASPARAGINASEs, only type II enzymes have been used in the treatment of acute lymphoblastic leukemia owing to their higher affinity for L-asparagine. However, current SCREENING media used for the isolation of L-ASPARAGINASE- producing microorganisms do not discriminate between the two types of L-ASPARAGINASE. During an OPTIMIZATION study conducted to increase L-ASPARAGINASE production by environmental HALOMONAS isolates, it was noticed that the pattern of L-ASPARAGINASE production in response to variations in glucose concentration varied between different isolates suggesting that they differ in their ability to produce type II L-ASPARAGINASEs, an observation that was confirmed by further experiments. Bioinformatics analysis of available HALOMONAS whole genome sequences revealed that indeed some species of this genus possess both L-ASPARAGINASE types while others possess only type I enzymes. By comparing the growth pattern of these isolates on different media, we propose that by omitting glucose, reducing the concentration of L-asparagine and providing an alternative nitrogen source in L-ASPARAGINASE SCREENING media it may be possible to differentiate between type I and type II activities.

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APA: Copy

SHARAFI, ZEINAB, BARATI, MAHMOOD, KHOSHAYAND, MOHAMMAD REZA, & ADRANGI, SINA. (2017). SCREENING FOR TYPE II L-ASPARAGINASES: LESSONS FROM THE GENUS HALOMONAS. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 16(4), 1565-1573. SID. https://sid.ir/paper/288722/en

Vancouver: Copy

SHARAFI ZEINAB, BARATI MAHMOOD, KHOSHAYAND MOHAMMAD REZA, ADRANGI SINA. SCREENING FOR TYPE II L-ASPARAGINASES: LESSONS FROM THE GENUS HALOMONAS. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2017;16(4):1565-1573. Available from: https://sid.ir/paper/288722/en

IEEE: Copy

ZEINAB SHARAFI, MAHMOOD BARATI, MOHAMMAD REZA KHOSHAYAND, and SINA ADRANGI, “SCREENING FOR TYPE II L-ASPARAGINASES: LESSONS FROM THE GENUS HALOMONAS,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 16, no. 4, pp. 1565–1573, 2017, [Online]. Available: https://sid.ir/paper/288722/en

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