SYNTHESIS OF LINEAR AND CYCLIC DISULFIDE HEPTAPEPTIDES OF LONGICALYCININ A AND EVALUATION OF TOXICITY ON CANCEROUS CELLS HEPG2 AND HT-29

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HOUSHDAR TEHRANI MOHAMMAD HASSAN; BAMONIRI ABDOLHAMID; MIRJALILI BIBI FATEMEH; GHOLIBEIKIAN MOHAMMADREZA

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Pharmaceutical Research Year:2018 | Volume:17 | Issue:3 Page(s): 956-963

Download Full-Text

View:

211

Download:

201

Cites:

Information Journal Paper

Title

SYNTHESIS OF LINEAR AND CYCLIC DISULFIDE HEPTAPEPTIDES OF LONGICALYCININ A AND EVALUATION OF TOXICITY ON CANCEROUS CELLS HEPG2 AND HT-29

Author(s)

HOUSHDAR TEHRANI MOHAMMAD HASSAN | BAMONIRI ABDOLHAMID | MIRJALILI BIBI FATEMEH | GHOLIBEIKIAN MOHAMMADREZA | Issue Writer Certificate

Keywords

LONGICALYCININ A ANALOGUE

DISULFIDE BAND

ANTICANCER

MTT ASSAY

APOPTOSIS

Abstract

In this work, linear and cyclic disulfide heptapeptides of Longicalycinin A have been successfully synthesized by solid phase methodology with Fmoc /t-Bu and solution phase, respectively.2-Chlorotrityl chloride resin (2-CTC) was used as a solid support. The synthesized linear disulfide analogue of Longicalycinin A was cleaved from the resin as a protected peptide. The final deprotection was performed by treatment with TFA 95% containing scavengers to achieve the deprotected linear disulfide analogue of Longicalycinin A which was characterized by different instrumental methods using LC-MS and FT-IR. Macrocyclization of deprotected linear peptide was done by an oxidating reagent. Linear and cyclic disulfide heptapeptides of Longicalycinin A were evaluated their toxic activity against cell lines of HepG2 and HT-29 using 3- (4, 5-dimethylthiazol-2-yl) -2, 5-diphenyltetrazolium bromide reagent in MTT ASSAY. The synthetic analogues showed a relative good activity against cell lines of HepG2 and HT- 29 with IC50 values from 10.33 mg/mL to 12.45 mg/mL, in comparison to the standard drug 5-fluorouracil (5-FU). Safety profiles of the synthesized linear and cyclic disulfide analogues of Longicalycinin A were also examined on skin fibroblast cells. Between the linear and cyclic disulfide heptapeptides of Longicalycinin A, the cyclic peptide showed a considerable toxic activity on the cancerous cell lines along with a low safety result on normal cells. Therefore, the linear disulfide heptapeptide of Longicalycinin A would be encouraging to develop new ANTICANCER agents.

Cites

No record.

References

No record.

Cite

APA: Copy

HOUSHDAR TEHRANI, MOHAMMAD HASSAN, BAMONIRI, ABDOLHAMID, MIRJALILI, BIBI FATEMEH, & GHOLIBEIKIAN, MOHAMMADREZA. (2018). SYNTHESIS OF LINEAR AND CYCLIC DISULFIDE HEPTAPEPTIDES OF LONGICALYCININ A AND EVALUATION OF TOXICITY ON CANCEROUS CELLS HEPG2 AND HT-29. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 17(3), 956-963. SID. https://sid.ir/paper/288779/en

Vancouver: Copy

HOUSHDAR TEHRANI MOHAMMAD HASSAN, BAMONIRI ABDOLHAMID, MIRJALILI BIBI FATEMEH, GHOLIBEIKIAN MOHAMMADREZA. SYNTHESIS OF LINEAR AND CYCLIC DISULFIDE HEPTAPEPTIDES OF LONGICALYCININ A AND EVALUATION OF TOXICITY ON CANCEROUS CELLS HEPG2 AND HT-29. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2018;17(3):956-963. Available from: https://sid.ir/paper/288779/en

IEEE: Copy

MOHAMMAD HASSAN HOUSHDAR TEHRANI, ABDOLHAMID BAMONIRI, BIBI FATEMEH MIRJALILI, and MOHAMMADREZA GHOLIBEIKIAN, “SYNTHESIS OF LINEAR AND CYCLIC DISULFIDE HEPTAPEPTIDES OF LONGICALYCININ A AND EVALUATION OF TOXICITY ON CANCEROUS CELLS HEPG2 AND HT-29,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 17, no. 3, pp. 956–963, 2018, [Online]. Available: https://sid.ir/paper/288779/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops