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Information Journal Paper

Title

Rapid Extracellular Biosynthesis of Silver Nanoparticles by Cunninghamella phaeospora Culture Supernatant

Pages

  915-924

Abstract

 The development of green approaches for the biosynthesis of Silver nanoparticles (AgNPs) is of prime significance in the field of nanotechnology research. A fast and eco-friendly protocol for the biosynthesis of extracellular AgNPs using culture supernatant (CS) from the fungus Cunninghamella phaeospora was studied in this work. This CS was proved as a potential new source for the extracellular biosynthesis of AgNPs. The AgNPs were formed at 100 oC and pH 9 within four min of contact between CS and 1mM silver nitrate (AgNO3) solution. Nitrate reductase (NR) was confirmed to play a pivotal role in the biosynthesis of AgNPs. The enzyme expressed its highest activity at 80 oC and pH 9. At 100 oC the enzyme retained 70% of its original activity for one hour. The half-life (T1/2) of the enzyme activity was calculated to be 1. 55 h confirming its thermostability. The produced A g NPs were characterized by UVVis spectroscopy, high resolution-transmission electron microscope (HR-TEM) a nd x-ray diffraction (XRD). These NPs showed an absorption peak at 415 nm in UV-Vis spectrum corresponding to the plasmon resonance of AgNPs. Transmission electron micrographs revealed the production of monodispersed spherical NPs with average particle size 14 nm. XRD spectrum of the NPs confirmed the formation of metallic crystalline silver. It was also suggested that the aromatic amino acids play a role in the biosynthesis process. The current research provided an insight on the green biosynthesis of AgNPs including some mechanistic aspects using a new mycogenic source.

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    APA: Copy

    Ghareib, Mohamed, Abu Tahon, Medhat, Mostafa Saif, Mona, & Abdallah, Wafaa El Sayed. (2016). Rapid Extracellular Biosynthesis of Silver Nanoparticles by Cunninghamella phaeospora Culture Supernatant. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 15(4), 915-924. SID. https://sid.ir/paper/289064/en

    Vancouver: Copy

    Ghareib Mohamed, Abu Tahon Medhat, Mostafa Saif Mona, Abdallah Wafaa El Sayed. Rapid Extracellular Biosynthesis of Silver Nanoparticles by Cunninghamella phaeospora Culture Supernatant. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2016;15(4):915-924. Available from: https://sid.ir/paper/289064/en

    IEEE: Copy

    Mohamed Ghareib, Medhat Abu Tahon, Mona Mostafa Saif, and Wafaa El Sayed Abdallah, “Rapid Extracellular Biosynthesis of Silver Nanoparticles by Cunninghamella phaeospora Culture Supernatant,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 15, no. 4, pp. 915–924, 2016, [Online]. Available: https://sid.ir/paper/289064/en

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