ASSESSMENT OF INDIRECT HEMAGGLUTINATION AND ZYMOGRAPHY PROCEDURES IN EVALUATION OF GELATINASE A IN PATIENTS WITH BENIGN AND MALIGNANT PROSTATE HYPERPLASIA

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AALIZADEH N.; PEZESHKI M.; KORRAMIZADEH M.R.; ESLAMI M.B.; ZERAATI H.A.

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Journal: Iranian Journal of Allergy, Asthma and Immunology Year:2003 | Volume:2 | Issue:3 Page(s): 159-163

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Title

ASSESSMENT OF INDIRECT HEMAGGLUTINATION AND ZYMOGRAPHY PROCEDURES IN EVALUATION OF GELATINASE A IN PATIENTS WITH BENIGN AND MALIGNANT PROSTATE HYPERPLASIA

Author(s)

Keywords

GELATINASE A

IHA

PSA

ZYMOGRAPHY

Abstract

There are increasing data on novel tumor markers such as GELATINASE A, which play a key role in tissue invasion and metastasis. Since prostate cancer is one of the common malignancies, we designed a simple and applicable Indirect Hemagglutination (IHA) test for determination of total GELATINASE A in serum samples. In this study, we have analyzed the circulating form of GELATINASE A (MMP-2) in patients suffering from either benign prostate hyperplasia (n= 54) or prostate cancer (n= 26) and normal individuals as control (n= 26). The gelatinolytic activity was determined by ZYMOGRAPHY followed by densitometric analysis. PSA was quantified by using a standard ELISA technique. Correlation of densitometric analysis of GELATINASE A activity and IHA titer was significant at 0.01 level (p< 0.01, r = 0.916). Correlation of PSA and IHA titer was significant at 0.01 level (p< 0.01, r = 0.746). Border line IHA titer in patients with prostate cancer was 512 ± 1 tube titer, in benign prostate hyperplasia patients was 128 ± 1 tube titer, and the titer in normal individuals was 8 ± 1 tube titer. These results demonstrate that IHA compared to ZYMOGRAPHY may be a better and simpler procedure in monitoring and screening patients with prostate cancer.

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APA: Copy

AALIZADEH, N., PEZESHKI, M., KORRAMIZADEH, M.R., ESLAMI, M.B., & ZERAATI, H.A.. (2003). ASSESSMENT OF INDIRECT HEMAGGLUTINATION AND ZYMOGRAPHY PROCEDURES IN EVALUATION OF GELATINASE A IN PATIENTS WITH BENIGN AND MALIGNANT PROSTATE HYPERPLASIA. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), 2(3), 159-163. SID. https://sid.ir/paper/291029/en

Vancouver: Copy

AALIZADEH N., PEZESHKI M., KORRAMIZADEH M.R., ESLAMI M.B., ZERAATI H.A.. ASSESSMENT OF INDIRECT HEMAGGLUTINATION AND ZYMOGRAPHY PROCEDURES IN EVALUATION OF GELATINASE A IN PATIENTS WITH BENIGN AND MALIGNANT PROSTATE HYPERPLASIA. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI)[Internet]. 2003;2(3):159-163. Available from: https://sid.ir/paper/291029/en

IEEE: Copy

N. AALIZADEH, M. PEZESHKI, M.R. KORRAMIZADEH, M.B. ESLAMI, and H.A. ZERAATI, “ASSESSMENT OF INDIRECT HEMAGGLUTINATION AND ZYMOGRAPHY PROCEDURES IN EVALUATION OF GELATINASE A IN PATIENTS WITH BENIGN AND MALIGNANT PROSTATE HYPERPLASIA,” IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), vol. 2, no. 3, pp. 159–163, 2003, [Online]. Available: https://sid.ir/paper/291029/en

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