EXPRESSION AND PURIFICATION OF A NOVEL COMPUTATIONALLY DESIGNED ANTIGEN FOR SIMULTANEOUSLY DETECTION OF HTLV-1 AND HBV ANTIBODIES

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HEYDARI ZARNAGH HAFEZ; RAVANSHAD MEHRDAD; POURFATHOLLAH ALI AKBAR; RASAEE MOHAMMAD JAVAD

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Journal Paper

Paper Information

Journal: Iranian Journal of Allergy, Asthma and Immunology Year:2015 | Volume:14 | Issue:2 Page(s): 168-178

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Title

EXPRESSION AND PURIFICATION OF A NOVEL COMPUTATIONALLY DESIGNED ANTIGEN FOR SIMULTANEOUSLY DETECTION OF HTLV-1 AND HBV ANTIBODIES

Author(s)

HEYDARI ZARNAGH HAFEZ | RAVANSHAD MEHRDAD | POURFATHOLLAH ALI AKBAR | RASAEE MOHAMMAD JAVAD | Issue Writer Certificate

Keywords

BLOOD SCREENING

COMPUTATIONAL ANALYSIS

HBV

HTLV-I

TRANSFUSION

Abstract

Computational tools are reliable alternatives to laborious work in chimeric protein design. In this study, a chimeric antigen was designed using computational techniques for simultaneous detection of anti-HTLV-I and anti-HBV in infected sera.Databases were searched for amino acid sequences of HBV/HLV-I diagnostic antigens. The immunodominant fragments were selected based on propensity scales. The diagnostic antigen was designed using these fragments. Secondary and tertiary structures were predicted and the B-cell epitopes were mapped on the surface of built model. The synthetic DNA coding antigen was sub-cloned into pGS21a expression vector.SDS-PAGE analysis showed that glutathione fused antigen was highly expressed in E. coli BL21 (DE3) cells. The recombinant antigen was purified by nickel affinity chromatography. ELISA results showed that soluble antigen could specifically react with the HTLV-I and HBV infected sera.This specific antigen could be used as suitable agent for antibody-antigen based screening tests and can help clinicians in order to perform quick and precise screening of the HBV and HTLV-I infections.

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APA: Copy

HEYDARI ZARNAGH, HAFEZ, RAVANSHAD, MEHRDAD, POURFATHOLLAH, ALI AKBAR, & RASAEE, MOHAMMAD JAVAD. (2015). EXPRESSION AND PURIFICATION OF A NOVEL COMPUTATIONALLY DESIGNED ANTIGEN FOR SIMULTANEOUSLY DETECTION OF HTLV-1 AND HBV ANTIBODIES. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), 14(2), 168-178. SID. https://sid.ir/paper/291424/en

Vancouver: Copy

HEYDARI ZARNAGH HAFEZ, RAVANSHAD MEHRDAD, POURFATHOLLAH ALI AKBAR, RASAEE MOHAMMAD JAVAD. EXPRESSION AND PURIFICATION OF A NOVEL COMPUTATIONALLY DESIGNED ANTIGEN FOR SIMULTANEOUSLY DETECTION OF HTLV-1 AND HBV ANTIBODIES. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI)[Internet]. 2015;14(2):168-178. Available from: https://sid.ir/paper/291424/en

IEEE: Copy

HAFEZ HEYDARI ZARNAGH, MEHRDAD RAVANSHAD, ALI AKBAR POURFATHOLLAH, and MOHAMMAD JAVAD RASAEE, “EXPRESSION AND PURIFICATION OF A NOVEL COMPUTATIONALLY DESIGNED ANTIGEN FOR SIMULTANEOUSLY DETECTION OF HTLV-1 AND HBV ANTIBODIES,” IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), vol. 14, no. 2, pp. 168–178, 2015, [Online]. Available: https://sid.ir/paper/291424/en

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