TRANSFORMING GROWTH FACTOR BETA-INDUCED IS ESSENTIAL FOR ENDOTOXIN TOLERANCE INDUCED BY A LOW DOSE OF LIPOPOLYSACCHARIDE IN HUMAN PERIPHERAL BLOOD MONONUCLEAR CELLS

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YANG YAN; SUN HANXIAO; LI XIUYING; DING QING; WEI PIJIN; ZHOU JINGGUANG

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Journal Paper

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Journal: Iranian Journal of Allergy, Asthma and Immunology Year:2015 | Volume:14 | Issue:3 Page(s): 321-330

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Title

TRANSFORMING GROWTH FACTOR BETA-INDUCED IS ESSENTIAL FOR ENDOTOXIN TOLERANCE INDUCED BY A LOW DOSE OF LIPOPOLYSACCHARIDE IN HUMAN PERIPHERAL BLOOD MONONUCLEAR CELLS

Author(s)

Keywords

ENDOTOXIN TOLERANCE

LIPOPOLYSACCHARIDE

PBMCS

TGFBI

Abstract

Our prior study found that transforming growth factor beta-induced (TGFBI) is an important negative regulator in TLR-induced inflammation. However, whether TGFBI may affect inflammation during LIPOPOLYSACCHARIDE (LPS)-induced ENDOTOXIN TOLERANCE (ET) is still unclear.This study aimed to investigate whether TGFBI was involved in the mechanisms of ET in human through dampening nuclear factor-kappa B (NF-xB) mediated pathway. ET models of isolated healthy volunteers peripheral blood mononuclear cells (PBMCs) were established by pretreating with a low dose of LPS to observe the changes of TGFBI expression during ET induction, compared with ten healthy controls. Moreover, a vector-based short hairpin RNA expression system was used to specifically inhibit TGFBI expression to further explore its role in ET induction. The expression was analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. The responses to LPS were determined by the activation of NF-xB, the production of tumor necrosis factor-a (TNF-a) and Nitric Oxide (NO), which were analysed by enzyme-linked immuno sorbent assay (ELISA).The results showed that TGFBI expression in the ET group obviously increased; ET also led to a hyporesponse of PBMCS to LPS with less activation of NF-xB, less production of TNF-a and NO, as well as more expression of TGFBI than those of non-ET group. Moreover, the inhibitory effect was partly refracted in plasmid TGFBI short hairpin RNA (pTGFBI-shRNA) transfected PBMCS. Meanwhile, the absence of TGFBI caused abnormal enhancement of inflammatory cytokine production and it was involved in ET induction through dampening NF-xB mediated pathway.Therefore, TGFBI may be a new target for the clinical treatment of inflammatory disorders.

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APA: Copy

YANG, YAN, SUN, HANXIAO, LI, XIUYING, DING, QING, WEI, PIJIN, & ZHOU, JINGGUANG. (2015). TRANSFORMING GROWTH FACTOR BETA-INDUCED IS ESSENTIAL FOR ENDOTOXIN TOLERANCE INDUCED BY A LOW DOSE OF LIPOPOLYSACCHARIDE IN HUMAN PERIPHERAL BLOOD MONONUCLEAR CELLS. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), 14(3), 321-330. SID. https://sid.ir/paper/291440/en

Vancouver: Copy

YANG YAN, SUN HANXIAO, LI XIUYING, DING QING, WEI PIJIN, ZHOU JINGGUANG. TRANSFORMING GROWTH FACTOR BETA-INDUCED IS ESSENTIAL FOR ENDOTOXIN TOLERANCE INDUCED BY A LOW DOSE OF LIPOPOLYSACCHARIDE IN HUMAN PERIPHERAL BLOOD MONONUCLEAR CELLS. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI)[Internet]. 2015;14(3):321-330. Available from: https://sid.ir/paper/291440/en

IEEE: Copy

YAN YANG, HANXIAO SUN, XIUYING LI, QING DING, PIJIN WEI, and JINGGUANG ZHOU, “TRANSFORMING GROWTH FACTOR BETA-INDUCED IS ESSENTIAL FOR ENDOTOXIN TOLERANCE INDUCED BY A LOW DOSE OF LIPOPOLYSACCHARIDE IN HUMAN PERIPHERAL BLOOD MONONUCLEAR CELLS,” IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), vol. 14, no. 3, pp. 321–330, 2015, [Online]. Available: https://sid.ir/paper/291440/en

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