Isolation of a novel Anti-KDR3 Single-chain Variable Fragment Antibody from a Phage Display Library

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Kordi Shirafkan; RAHMATI YAMCHI MOHAMMAD; ASGHARI VOSTAKOLAEI MEHDI; ETEMADI ALI; BARZEGARI ABOLFAZL; ABDOLALIZADEH JALAL

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Journal Paper

Paper Information

Journal: Iranian Journal of Allergy, Asthma and Immunology Year:2019 | Volume:18 | Issue:3 Page(s): 289-299

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Title

Isolation of a novel Anti-KDR3 Single-chain Variable Fragment Antibody from a Phage Display Library

Author(s)

Keywords

Monoclonal antibody

Kinase insert domain receptor (KDR) 3 Phage display

Single-chain variable fragment (scFv)

Vascular endothelial growth factor receptor-2 (VEGFR2)

Abstract

Vascular endothelial growth factor receptor 2 (VEGFR-2) is known as one of the important antigens playing a vital role in angiogenesis. In this study, phage display technology (PDT) was used to produce a single-chain variable fragment (scFv) antibody against a region of the domain 3 in VEGFR-2 called kinase insert domain receptor 3 (KDR3). After designing the KDR3 peptide and biopanning, a colony was chosen for scFv antibody expression. Following expression and purification; western blotting, dot blotting and immunofluorescence (IF) were used to evaluate the antibody function. Surface plasmon resonance (SPR) was also employed to measure affinity of produced antibody. Once a colony was selected and transferred to the expression host, the scFv antibody was expressed in the expected range of 28 kDa. Using a designed chromatography column, antibody purification was found to be about 95%. In this study, a novel scFv with the capability of binding to KDR3 was isolated and purified and its intracellular function was investigated and verified.

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APA: Copy

Kordi, Shirafkan, RAHMATI YAMCHI, MOHAMMAD, ASGHARI VOSTAKOLAEI, MEHDI, ETEMADI, ALI, BARZEGARI, ABOLFAZL, & ABDOLALIZADEH, JALAL. (2019). Isolation of a novel Anti-KDR3 Single-chain Variable Fragment Antibody from a Phage Display Library. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), 18(3), 289-299. SID. https://sid.ir/paper/291578/en

Vancouver: Copy

Kordi Shirafkan, RAHMATI YAMCHI MOHAMMAD, ASGHARI VOSTAKOLAEI MEHDI, ETEMADI ALI, BARZEGARI ABOLFAZL, ABDOLALIZADEH JALAL. Isolation of a novel Anti-KDR3 Single-chain Variable Fragment Antibody from a Phage Display Library. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI)[Internet]. 2019;18(3):289-299. Available from: https://sid.ir/paper/291578/en

IEEE: Copy

Shirafkan Kordi, MOHAMMAD RAHMATI YAMCHI, MEHDI ASGHARI VOSTAKOLAEI, ALI ETEMADI, ABOLFAZL BARZEGARI, and JALAL ABDOLALIZADEH, “Isolation of a novel Anti-KDR3 Single-chain Variable Fragment Antibody from a Phage Display Library,” IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), vol. 18, no. 3, pp. 289–299, 2019, [Online]. Available: https://sid.ir/paper/291578/en

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