Anti-inflammatory property of β-D-mannuronic acid (M2000) on expression and activity of matrix metalloproteinase-2 and-9 through CD147 molecule in phorbol myristate acetate-differentiated THP-1 cells

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Farahani Mohadese M.; MOTEVASELI ELAHE; Maghsood Faezeh; Heidari Kharaji Maryam; MIRSHAFIEY ABBAS

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Journal: Iranian Journal of Allergy, Asthma and Immunology Year:2017 | Volume:16 | Issue:5 Page(s): 443-451

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Title

Anti-inflammatory property of β-D-mannuronic acid (M2000) on expression and activity of matrix metalloproteinase-2 and-9 through CD147 molecule in phorbol myristate acetate-differentiated THP-1 cells

Author(s)

Keywords

CD147

Mannuronic acid

Matrix metalloproteinases

Tissue inhibitor of matrix metalloproteinase

Abstract

The aim of this study was to evaluate the effects of M2000, a novel non-steroidal anti-inflammatory drug (NSAID) with immunosuppressive property and without gastro-nephrotoxicitic effects on Matrix metalloproteinases (MMP)-2 and (MMP)-9 in phorbol myristate acetate (PMA)-differentiated THP-1 cells. Gene expression and activity of MMP-2 and MMP-9 are inhibited respectively by the Tissue inhibitor of matrix metalloproteinase (TIMP)-2 and (TIMP)-1 and are induced by extracellular matrix metalloproteinase inducer (CD147/EMMPRIN). In this study, real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to determine gene expression of MMP-2, MMP-9, TIMP-1, and TIMP-2. Flow cytometry and zymography were applied to determine cellular surface expression of CD147 and activity of MMP-2 and MMP-9, respectively. Our results showed that treatment of THP-1 cells with high concentration (25 μ g/mL) of M2000 significantly decreased the cellular surface expression of CD147 (p<0. 05) and the gene expression of MMP-2, MMP-9 and TIMP-1 (p<0. 05), and inhibited the gelatinolytic activity of MMP-2 and MMP-9 (p<0. 05). According to our results, M2000 can reduce inflammation through inhibition of the cellular surface expression of CD147 and decrease the gene expression and gelatinolytic activity of MMP-2 and MMP-9 in PMA-differentiated THP-1 cells.

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APA: Copy

Farahani, Mohadese M., MOTEVASELI, ELAHE, Maghsood, Faezeh, Heidari Kharaji, Maryam, & MIRSHAFIEY, ABBAS. (2017). Anti-inflammatory property of β-D-mannuronic acid (M2000) on expression and activity of matrix metalloproteinase-2 and-9 through CD147 molecule in phorbol myristate acetate-differentiated THP-1 cells. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), 16(5), 443-451. SID. https://sid.ir/paper/291630/en

Vancouver: Copy

Farahani Mohadese M., MOTEVASELI ELAHE, Maghsood Faezeh, Heidari Kharaji Maryam, MIRSHAFIEY ABBAS. Anti-inflammatory property of β-D-mannuronic acid (M2000) on expression and activity of matrix metalloproteinase-2 and-9 through CD147 molecule in phorbol myristate acetate-differentiated THP-1 cells. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI)[Internet]. 2017;16(5):443-451. Available from: https://sid.ir/paper/291630/en

IEEE: Copy

Mohadese M. Farahani, ELAHE MOTEVASELI, Faezeh Maghsood, Maryam Heidari Kharaji, and ABBAS MIRSHAFIEY, “Anti-inflammatory property of β-D-mannuronic acid (M2000) on expression and activity of matrix metalloproteinase-2 and-9 through CD147 molecule in phorbol myristate acetate-differentiated THP-1 cells,” IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), vol. 16, no. 5, pp. 443–451, 2017, [Online]. Available: https://sid.ir/paper/291630/en

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