A STUDY ON IN-VITRO TRANS DIFFERENTIATION OF RAT BONE MARROW STROMAL CELLS INTO NEUROEPITHELIAL-LIKE CELLS

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KAKA GH.R.; TIRAIHI T.; ARAB KHERADMAND A.; AZIZZADEH DELSHAD A.R.

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Journal Paper

Paper Information

Journal: Iranian Red Crescent Medical Journal Year:2009 | Volume:11 | Issue:2 Page(s): 133-139

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Title

A STUDY ON IN-VITRO TRANS DIFFERENTIATION OF RAT BONE MARROW STROMAL CELLS INTO NEUROEPITHELIAL-LIKE CELLS

Author(s)

KAKA GH.R. | TIRAIHI T. | ARAB KHERADMAND A. | AZIZZADEH DELSHAD A.R. | Issue Writer Certificate

Keywords

RAT

BMSCS

NEUROEPITHELIAL-LIKE CELLS

TRANSDIFFERENTIATION

Abstract

Background: Bone marrow stem cells (BMSCs) are a rich source of stem cells and may represent a valid alternative to neural or embryonic stem cells by replacing the autologous damaged tissues in neurodegeneRATive diseases. In this study, we attempted to devise a protocol for the induction of BMSCS into NEUROEPITHELIAL-LIKE CELLS (NELCs).Methods: RAT BMSCS were isolated from the long bones of adult Sprague–Dawley RATs. Their purity in the 4th passage was evaluated with fibronectin by immunocytochemistry, and the stemness marker Oct-4 was assessed by RT-PCR technique. The cells were expanded and induced in the induction stage. The BMSCS were incubated with either b-mercaptoethanol (bME) (1 mM), dimethyl sulfoxide (DMSO) (2%) or biotylated hydroxyanisol or butylated hydroxyanisol (BHA) (200 mM) in a-MEM medium without fetal bovine serum (FBS). They were washed with phosphate buffer saline (PBS) and proceeded to the 2nd phase of induction, where the induction medium was changed with a-MEM and 15% FBS containing all-trans retinoic acid (RA) (1 mM) (for 3 days). Then, the expression of the markers was assessed with GFAP, nestin and neurofilament 68 antibodies, respectively and the expression of Oct-4 and NeuroD was evaluated by RT-PCR. Results: The purity of the BMSCS at the 4th passage was more than 92%. The mRNA of Oct-4 was expressed in these cells. Induction of BMSCS by DMSO-RA could differentiate NELCs significantly more than bME-RA and BHA-RA. The TRANSDIFFERENTIATION of NELCs was evaluated by nestin antibody and NeuroD mRNA expression; later markers expressed very low detectable level in BMSCS. But the differentiation of BMSCS into astrocytes was less in all of the experiment groups that is estimated GFAP antibody.Conclusion: The application of DMSO-RA can transdifferentiate BMSCS into NELCs in- vitro.

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APA: Copy

KAKA, GH.R., TIRAIHI, T., ARAB KHERADMAND, A., & AZIZZADEH DELSHAD, A.R.. (2009). A STUDY ON IN-VITRO TRANS DIFFERENTIATION OF RAT BONE MARROW STROMAL CELLS INTO NEUROEPITHELIAL-LIKE CELLS. IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ), 11(2), 133-139. SID. https://sid.ir/paper/292123/en

Vancouver: Copy

KAKA GH.R., TIRAIHI T., ARAB KHERADMAND A., AZIZZADEH DELSHAD A.R.. A STUDY ON IN-VITRO TRANS DIFFERENTIATION OF RAT BONE MARROW STROMAL CELLS INTO NEUROEPITHELIAL-LIKE CELLS. IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ)[Internet]. 2009;11(2):133-139. Available from: https://sid.ir/paper/292123/en

IEEE: Copy

GH.R. KAKA, T. TIRAIHI, A. ARAB KHERADMAND, and A.R. AZIZZADEH DELSHAD, “A STUDY ON IN-VITRO TRANS DIFFERENTIATION OF RAT BONE MARROW STROMAL CELLS INTO NEUROEPITHELIAL-LIKE CELLS,” IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ), vol. 11, no. 2, pp. 133–139, 2009, [Online]. Available: https://sid.ir/paper/292123/en

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