CONVENTIONAL AGAR-BASED CULTURE METHOD, AND NUCLEIC ACID AMPLIFICATION TEST (NAAT) OF THE CPPB GENE FOR DETECTION OF NEISSERIA GONORRHEA IN PREGNANT WOMEN ENDOCERVICAL SWAB SPECIMENS

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HASSANZADEH PARVIN; MARDANEH JALAL; MOTAMEDIFAR MOHAMMAD

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Journal: Iranian Red Crescent Medical Journal Year:2013 | Volume:15 | Issue:3 Page(s): 207-211

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Title

CONVENTIONAL AGAR-BASED CULTURE METHOD, AND NUCLEIC ACID AMPLIFICATION TEST (NAAT) OF THE CPPB GENE FOR DETECTION OF NEISSERIA GONORRHEA IN PREGNANT WOMEN ENDOCERVICAL SWAB SPECIMENS

Author(s)

HASSANZADEH PARVIN | MARDANEH JALAL | MOTAMEDIFAR MOHAMMAD | Issue Writer Certificate

Keywords

PREGNANT WOMEN

NEISSERIA GONORRHEA

CULTURE

NUCLEIC ACID AMPLIFICATION TECHNIQUES (NAAT)

Abstract

Background: NEISSERIA GONORRHEA is the etiological agent of the sexually transmitted disease (STD) gonorrhea, and primarily infects the mucous membranes of the urethra, end ocervix, pharynx or rectum of females which may result in substantial morbidity. N. gonorrhea also causes disseminated infection, with complications that may result in ectopic pregnancy, tubal infertility, chronic pelvic pain or maternal transmission of gonorrhea, and also increases susceptibility to HIV.Objectives: In the present investigation, we used conventional agar-based CULTURE method, and nucleic acid amplification of CCPB gene for detection of NEISSERIA GONORRHEA in end ocervical swabs samples collected from PREGNANT WOMEN studied.Patients and Methods: End ocervical swabs specimens for this study were obtained from 1100 PREGNANT WOMEN who presented to Shiraz (Iran) Hospitals from 2009 to 2011. In the present investigation we used conventional agar-based CULTURE method, and nucleic acid amplification test (NAAT) of CCPB gene for detection of NEISSERIA GONORRHEA in end ocervical swabs samples collected from PREGNANT WOMEN studied. From each pregnant woman two end ocervical swabs were taken: one swab placed in tubes containing phosphate buffered saline for Polymerase Chain Reaction, and the other to inoculate on CULTURE media.Results: Among 1100 end ocervical swabs examined, 13 (1.18%) samples had positive results by polymerase chain reaction (PCR) on NEISSERIA GONORRHEA CCPB gene. All end ocervical swabs CULTURE had negative results for NEISSERIA GONORRHEA. 84 (7%) of the women had vaginal discharge, in whom PCR on end ocervical swabs of these individuals had negative findings.Conclusions: Nucleic acid amplification tests (NAATs) are very appropriate in detection of infected individuals. Detection techniques such as NAATs are independent of bacterial viability, and have a potential to limit false negative samples, therefore, in our country, the application of different laboratory diagnosis methods including NAATs with CULTURE as gold standard for determination antimicrobial susceptibility is essential.

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APA: Copy

HASSANZADEH, PARVIN, MARDANEH, JALAL, & MOTAMEDIFAR, MOHAMMAD. (2013). CONVENTIONAL AGAR-BASED CULTURE METHOD, AND NUCLEIC ACID AMPLIFICATION TEST (NAAT) OF THE CPPB GENE FOR DETECTION OF NEISSERIA GONORRHEA IN PREGNANT WOMEN ENDOCERVICAL SWAB SPECIMENS. IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ), 15(3), 207-211. SID. https://sid.ir/paper/292837/en

Vancouver: Copy

HASSANZADEH PARVIN, MARDANEH JALAL, MOTAMEDIFAR MOHAMMAD. CONVENTIONAL AGAR-BASED CULTURE METHOD, AND NUCLEIC ACID AMPLIFICATION TEST (NAAT) OF THE CPPB GENE FOR DETECTION OF NEISSERIA GONORRHEA IN PREGNANT WOMEN ENDOCERVICAL SWAB SPECIMENS. IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ)[Internet]. 2013;15(3):207-211. Available from: https://sid.ir/paper/292837/en

IEEE: Copy

PARVIN HASSANZADEH, JALAL MARDANEH, and MOHAMMAD MOTAMEDIFAR, “CONVENTIONAL AGAR-BASED CULTURE METHOD, AND NUCLEIC ACID AMPLIFICATION TEST (NAAT) OF THE CPPB GENE FOR DETECTION OF NEISSERIA GONORRHEA IN PREGNANT WOMEN ENDOCERVICAL SWAB SPECIMENS,” IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ), vol. 15, no. 3, pp. 207–211, 2013, [Online]. Available: https://sid.ir/paper/292837/en

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